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Md Zannat Ali¹, Sankaranarayanan Srinivasan², Selina Akter³

¹Department of Microbiology, Jashore University of Science and Technology, Jashore, Bangladesh.

Scientific reports

|March 12, 2024

概括

多种药物耐药性,特别是扩展光谱β-乳糖酶 (ESBL) 生产细菌,可以通过纸币传播. 在孟加拉国纸币上发现的CTX-M突变可能导致梅西林纳姆耐药性.

科学领域:

微生物学微生物学
分子生物学分子生物学
药物耐药性药物耐药性药物耐药性

背景情况:

扩展光谱β-乳糖酶 (ESBL) 生产生物体是挑战性感染的重要原因.
酶的CTX-M家族是一个快速增长的,在全球分布的群体,在Enterobacteriaceae中发现.
由于经常流通,纸币是传播多药耐药性的潜在载体.

研究的目的:

在孟加拉国纸币上选ESBL携带的细菌.
为了识别和描述这些纸币上存在的CTX-M突变.
调查这些突变对抗生素耐药性的潜在影响,特别是对美西林的抗生素耐药性.

主要方法:

从纸币上选携带ESBL的细菌.
CTX-M 基因的基因测序.
使用瑞士模型对CTX-M变体的同质模型建模.
使用Autodock 4.2与野生类型和突变的CTX-M模型进行分子对接.
使用PyMOL进行分子相互作用的视觉检查.

主要成果:

部分测序的blaCTX-M基因与blaCTX-M-10和blaCTX-M-15有关.
已识别的单核酸替代突变:G613T (静音),A626T (I176F) 和A503G (N135D). 这些突变包括:
同样性建模证实与模板的高度相似性;突变残留物 (Asp135,Phe176) 保持了类似的方向.
分子对接预测了梅西林与Lys237,Ser240和Asp135残留物的结合,最低的结合能量在Asp135.5.

结论:

在孟加拉国纸币上发现的CTX-M突变与blaCTX-M-15和blaCTX-M-10有关.
N135D和I176F突变可能会影响梅西林胺结合.
这些发现支持了CTX-M突变导致梅西林胺耐药性的假设,这可能与CTX-M-15转换为CTX-M-127有关.

关键词:

抗生素耐药性抗生素耐药性银行纸币纸币纸币在CTX-M-127中使用.在CTX-M-15中使用.扩展光谱β-lactamase是一种扩展光谱β-lactamase.突变突变是一种突变.

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