在Vigna Savi (Leguminosae) 子属中细胞分子的多样性
Sibelle Dias1, Rosilda Cintra Souza1,2, Emanuelle Varão Vasconcelos1
1Departamento de Genética, Centro de Biociências, Universidade Federal de Pernambuco, Recife, PE, Brazil.
Protoplasma
|March 12, 2024
概括
这项研究揭示了维格纳属6号染色体上保存的祖先35SrDNA,尽管rDNA位点的演化多样化. 基因组大小保持不变,与35S rDNA复制数相关.
科学领域:
- 植物遗传学 植物遗传学
- 细胞遗传学 细胞遗传学
- 进化生物学是进化的生物学.
背景情况:
- 维格纳属 (Vigna) 是类植物科 (Leguminosae) 家族的一部分,包含大约150种,分别分布在五个亚属中.
- 了解维格纳内的型多样性和进化对于豆类遗传学至关重要.
研究的目的:
- 为了研究Vigna.属的型多样性和进化.
- 分析核糖体DNA (rDNA) 位点和基因组大小的分布和演变.
主要方法:
- 利用细胞遗传技术和DNA内容分析.
- 在染色体上使用细菌人造染色体光在位杂交 (BAC-FISH).
主要成果:
- 观察到35S rDNA模式的随机分布,副本数量从一个到七对跨子类的变化.
- 发现保存的5SrDNA拷贝数 (一对或两对),在Vigna radiata中例外.
- 在该属内注意到保存的基因组大小 (0.430.70 pg),DNA含量和35S rDNA位点数之间存在正相关性.
- 鉴定了染色体6上保存的祖先35SrDNA位点,表明其在多样化中的作用.
结论:
- 6号染色体上祖先的35SrDNA位点在Vigna中似乎得到了保留,尽管rDNA位点数和位置的显著多样化.
- 在维格纳进化过程中,保存的位置可能是扩散额外rDNA位点的来源.
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