Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

50.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Discrepancy-Guided Complementary Fusion for Unsupervised Multimodal Anomaly Detection.

Sensors (Basel, Switzerland)·2026
Same author

Weighted knowledge distillation for semi-supervised segmentation of maxillary sinus in panoramic X-ray images.

Scientific reports·2026
Same author

Position-wise comparison of handheld 6-lead ECG versus the standard 12-lead ECG in patients with arrhythmia: comparative study.

BMC cardiovascular disorders·2026
Same author

Spatial assessment of heat risk from deregulation of greenbelt in Seoul, South Korea.

Scientific reports·2026
Same author

Spatial-Semantic Object Relation Graph Networks for Vehicle Attachment Detection in Automatic Car Wash System.

Sensors (Basel, Switzerland)·2026
Same author

A hybrid piggyBac AAV-transposon and LNP-transposase for treating arginase deficiency: A 100-fold dose reduction compared to AAV alone.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

相关实验视频

Updated: Jul 1, 2025

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.0K

使用CRISPR-Cas12a进行单核酸微生物基因组编辑

Ho Joung Lee1, Sang Jun Lee2

  • 1Department of Systems Biotechnology, and Institute of Microbiomics, Chung-Ang University, Anseong, Republic of Korea.

Methods in molecular biology (Clifton, N.J.)
|March 12, 2024
PubMed
概括
此摘要是机器生成的。

研究人员使用改造的CRISPR-Cas12a系统和突变性DNA捐赠者在大肠杆菌中实现了精确的单核酸基因组编辑. 这种方法可以在微生物细胞中确切地进行基因修饰.

关键词:
3′-截断的crRNARNA可以使用.精确的基因组编辑.一个单基底的单基底.这就是Cas12a.

更多相关视频

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

33.9K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.6K

相关实验视频

Last Updated: Jul 1, 2025

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.0K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

33.9K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.6K

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 微生物基因组编辑对于合成生物学和生物技术至关重要.
  • 现有的方法,如捐赠者DNA定向突变发生和CRISPR-Cas12a在精度上有局限性.
  • 微生物编辑中的单核酸分辨率对于精确的基因操纵是非常理想的.

研究的目的:

  • 开发一种用于E. coli单核酸水平基因组编辑的方法.
  • 调查crRNA截断在CRISPR-Cas12a中介编辑中的作用.
  • 在特定的基因组位置实现精确的替代和indels.

主要方法:

  • 为了精确的编辑,采用了致变性DNA寡核酸供体.
  • 采用了使用截断crRNAs的CRISPR-Cas12a系统.
  • 针对大肠杆菌基因组中的galK基因进行编辑.

主要成果:

  • 在大肠杆菌基因组中成功实现了单核酸替代和indels.
  • 证明了crRNA 3'-end的最大切断可以提高Cas12a介导的编辑精度.
  • 在galK locus.展示了精确的编辑.

结论:

  • 在大肠杆菌中,使用CRISPR-Cas12a进行高保真性单核酸编辑时,最大限度的crRNA截断是关键.
  • 这种方法使微生物基因组的精确基因工程成为可能.
  • 开发的方法为微生物菌株发展和功能基因组学提供了强大的工具.