VPBrowse:基于MS/MS光谱的基因组表示,以量化10,000个牛蛋白
Selvam Paramasivan1,2, Mohamed Ashick3, Kevin J Dudley2
1School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Proteomics
|March 12, 2024
概括
一个新的在线平台,兽医蛋白质组浏览器 (VPBrowse),通过提供一个全面的光谱库来增强牛的定量蛋白质组学. 此资源有助于识别新的蛋白质生物标记物,如PSMA4,用于动物健康研究.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 生物信息学是一种生物信息学.
- 兽医科学 兽医科学 兽医科学
背景情况:
- SWATH获取是一种强大的定量蛋白质组学技术.
- 它在非实验室动物中的应用受到公共光谱图书馆和用户友好的平台缺乏限制.
- 这阻碍了兽医研究中的全面蛋白质组分析.
研究的目的:
- 开发一个在线平台,兽医蛋白质组浏览器 (VPBrowse),用于Bos taurus蛋白质组.
- 为兽医蛋白质组学中量化质谱测试创建一个全面和可访问的资源.
- 促进在动物健康研究中发现新型蛋白质生物标志物.
主要方法:
- 开发VPBrowse (http://browser.proteo.cloud/) 作为一个基于基因组的交互式在线平台.
- 包括超过36000个蛋白型的高质量碎片化光谱和超过10,000个蛋白质的实验证据.
- 数据以多种格式下载,并将其标准化为iRT尺度,以便与SWATH数据处理软件和各种染色学系统兼容.
主要成果:
- VPBrowse 1.0为Bos taurus蛋白质组探索提供了一个强大的数据库.
- 该平台促进了从已发表的血数据集中对其他蛋白质的无标签量化.
- 确定了PSMA4作为一种潜在的生物标志物,用于动物对剥角相关损伤的反应.
结论:
- VPBrowse显著提高了非实验室动物,特别是牛的定量蛋白质组学能力.
- 该平台支持识别与动物福利和健康相关的新型蛋白质生物标志物.
- VPBrowse是兽医蛋白质组研究和生物标志物发现的有价值,可访问的资源.
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