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库尔库调节NOX基因表达和ROS通过TGF-β激活肝细胞中的P-Smad3C产生ROS

Shahla Asadizade¹, Mahdi Hatami¹, Samaneh Salehipour Bavarsad¹

¹Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Iranian biomedical journal

|March 12, 2024

概括

黄素通过降低NADPH氧化酶 (NOX) 和活性氧物种 (ROS) 水平,有效降低肝纤维化. 这种治疗潜力与抑制肝星细胞 (HSC) 中Smad3酸化有关.

科学领域:

肝病学肝病学是一种肝病学.
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

肝纤维化涉及肝星细胞 (HSC) 和由于损伤而导致的肝功能受损.
在肝损伤期间,NADPH氧化酶 (NOXs) 1,2和4有助于活性氧物种 (ROS) 的产生,促进纤维化.
黄素显示出缓解肝纤维化的潜力.

研究的目的:

为了研究黄素对降低肝纤维化中的NOX和ROS水平的影响.
通过Smad3酸化抑制来研究黄素对NOX和ROS度的影响.
评估黄素在转化增长因子β (TGF-β) 激活的人类HSC中的治疗潜力.

主要方法:

用MTT测定评估了黄素对HSC的细胞毒性作用.
用TGF-β激活HSC,然后用不同度的黄素 (25-150μM) 进行治疗.
基因和蛋白质表达水平的NOX1,NOX2,NOX4,酸化Smad3C,α-平滑肌肌动蛋白 (α-SMA),和原1-α使用RT-PCR和西方涂抹评估.

主要成果:

黄素治疗 (100μM) 显著降低了NOX,α-SMA,原1-α和ROS水平的mRNA表达.
黄素显著降低了TGF-β激活的HSC中的p-Smad3C蛋白水平 (75μM时3的折叠变化和100μM时2的折叠变化).

结论:

黄素有效降低ROS,NOX,α-SMA和原1-α水平,表明它具有抗纤维性质.
该机制涉及p-Smad3C的抑制,这表明黄素在黄素的治疗作用中起着关键作用.
黄素显示出作为治疗肝纤维化的有效治疗剂的前景.

关键词:

库尔库明是一种黄素.肝硬化是肝硬化的一种疾病.在NADPH氧化酶中,NADPH具有氧化作用.有反应性氧物种的反应性氧物种.

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