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转录基因组概况显示,超排卵与淋巴激素释放激素激素激素激活剂触发了小鼠受损的胚胎植入

Meng Li¹, Jingmei Han², Nana Yang¹

¹College of Animal Science and Technology, Hebei Technology Innovation Center of Cattle and Sheep Embryo, Hebei Agricultural University, Baoding, China.

Frontiers in endocrinology

|March 12, 2024

概括

激素释放激素激素激素 (GnRHa) 触发与PMSG用于超排卵受损胚胎植入小鼠. 这是由于子宫雌激素反应过度,改变基因表达,导致异常的子宫内膜重塑和炎症增加.

科学领域:

生殖生物学生殖生物学
辅助生殖技术是一种辅助生殖技术.
分子内分泌学分子内分泌学

背景情况:

超排卵是辅助生殖的关键.
人类胆性腺激素 (hCG) 触发超排卵,但有卵巢过度刺激的风险.
淋巴激素释放激素激动剂 (GnRHa) 是另一种触发剂,但与更高的怀孕失败率有关.

研究的目的:

用小鼠模型研究GnRHa触发对胚胎植入的影响.
为了比较GnRHa触发的超排卵和自然周期之间的生殖结果.

主要方法:

小鼠接受了PMSG,其次是GnRHa (PG组) 或自然交配 (对照组,CTR).
卵子细胞,胚芽细胞,红色体和植入点的数量被量化.
卵巢,子宫和血清使用qRT-PCR,转录组测序和激素测定进行了分析.

主要成果:

PG组产生了更多的卵细胞,胚胎细胞和红色体.
然而,PG组显示白血病抑制因子 (Lif) mRNA表达减少,植入部位较少.
超排卵增加了雌激素分泌,导致异常的子宫内膜和细胞外基质重塑,改变了离子运输,并增加了与炎症相关的基因表达.

结论:

PMSG和GnRHa触发剂组合会损害小鼠的胚胎植入.
对超生理性雌激素水平的过度子宫反应会改变子宫内膜基因表达,离子运输和免疫反应.
这些分子变化有助于植入失败.

关键词:

作为性腺激素释放激素的激动剂.卵巢卵巢是一个卵巢.超级排卵是一种超级排卵.转录组 (transcriptome) 是一个转录组.子宫的感受性子宫的感受性

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