树提取物调节生物钟基因表达的昼夜节律 in vitro 和小鼠的轮子运行活动
Atsushi Haraguchi1, Yao Du1, Rena Shiraishi2
1Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University, Shinjuku, Tokyo, 162-8480 Japan.
Sleep and biological rhythms
|March 12, 2024
概括
树提取物及其代谢物,urolithin A和酸,调节细胞中昼夜钟基因节律,并改善老年小鼠的活性. 这表明树提取物是生物钟的新型调节者.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 老年学是一门学科.
背景情况:
- 上神核 (SCN) 是哺乳动物的中心昼夜时钟,调节日常节律.
- 衰老对昼夜节律和运动活动产生负面影响.
- 树提取物已知可以改善与年龄相关的生理衰退,但其对昼夜系统的影响尚不清楚.
研究的目的:
- 研究树提取物及其代谢物对昼夜钟基因表达的影响.
- 评估对年轻和老年小鼠运动活动节律的影响.
主要方法:
- 在小鼠胚胎纤维细胞 (MEF) 和SCN扩展体中检查了时钟基因表达节奏 (PER2::LUC).
- 给细胞和小鼠服用树提取物,基酸 (EA) 和尿酸氨酸 (ULT).
- 评估了年轻和老年小鼠的运动运动活动.
主要成果:
- 在EA和ULT治疗中,相延迟的PER2::LUC节律在SCN实验室中.
- 根据剂量,ULT延长了该周期,并增加了MEF中PER2::LUC节律的幅度.
- 树提取物在老老鼠中延长了活动时间,增加了车轮运行行为.
结论:
- 树提取物及其代谢物在体外影响昼夜钟基因表达.
- 树提取物显示出其潜力,可以调节昼夜节律和活体活动,特别是在老年人中.
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