在临床细菌分离物中扩展光谱β-乳酸酶的等离子体编码基因重复
Annika Sobkowiak1,2, Natalie Scherff1, Franziska Schuler3
1Institute of Hygiene, University Hospital Münster, Münster, Germany.
Frontiers in cellular and infection microbiology
|March 12, 2024
概括
在等离子体上的基因重复显著增加了细菌的抗微生物耐药性,如大肠杆菌和肺炎. 这种IS介导的转换机制驱动了扩展谱β-乳糖酶 (ESBL) 基因的传播,并增加了对抗生素的耐药性.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 一个健康,一个健康.
背景情况:
- 产生扩展光谱β-乳酸酶 (ESBL) 的肠道细菌构成了严重的全球健康威胁.
- 了解驱动抗菌素耐药性 (AMR) 的遗传机制对于有效的治疗策略至关重要.
研究的目的:
- 为了研究等离子体编码的ESBL基因复制.
- 为了确定这些重复对AMR表型在临床隔离物的影响.
主要方法:
- 从住院患者身上收集了多种耐药细菌.
- 进行长读全基因组测序以提取基因型.
- 描述了等离子体和移动遗传元件,将ESBL基因与塞夫塔齐最小抑制度 (MIC) 相关联.
主要成果:
- 在*Escherichia coli*和*Klebsiella pneumoniae*分离物中发现了四例用等离子体编码的ESBL基因重复.
- 发现了负责基因重复的插入序列 (IS) 介导的转换机制.
- 与单个副本基因相比,具有重复ESBL基因的隔离体显示出明显更高的ceftazidime MIC.
结论:
- 通过ESBL基因重复,可以增强对Ceftazidime的表型耐药性.
- 通过IS介导的ESBL基因转移是AMR发展和等离子体微观进化的关键机制.
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