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相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 1, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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PMAT:一个高效的植物线粒基因组组装工具包,使用低覆盖率的HiFi测序数据.

Changwei Bi1,2, Fei Shen3, Fuchuan Han4

  • 1State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Tree Genetics and Biotechnology of Educational Department of China, Key Laboratory of Tree Genetics and Silvicultural Sciences of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China.

Horticulture research
|March 12, 2024
PubMed
概括

一个新的工具包,PMAT,使用低覆盖率的HiFi测序数据有效地组装完整的植物线粒体基因组 (线粒体基因组). 这通过克服以前的组装挑战,推进了植物进化研究和育种应用.

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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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相关实验视频

Last Updated: Jul 1, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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科学领域:

  • 基因组学就是基因组学.
  • 植物科学 植物科学
  • 生物信息学是一种生物信息学.

背景情况:

  • 植物线粒体基因组 (线粒体基因组) 对于理解植物生物学,进化和繁殖至关重要.
  • 由于重组和基因转移,组装完整的植物线粒基因组是困难的.
  • 现有的测序方法在准确性,完整性和成本方面存在局限性.

研究的目的:

  • 开发一种有效的工具包,用于植物线粒基因组的新组装.
  • 利用低覆盖率的HiFi测序数据来改进线粒基因组组装.
  • 克服当前植物线粒体基因组组装方法的局限性.

主要方法:

  • 开发植物线粒基因组组装工具包 (PMAT).
  • 应用PMAT用于新组装13种不同的植物线粒基因组.
  • 评估PMAT的性能与现有的有机细胞基因组组装器相比.

主要成果:

  • 与现有工具相比,PMAT实现了更高的组装精度和完整性.
  • 通过仅使用1次HiFi测序数据,获得了完整的植物线粒体.
  • 该工具包证明了低覆盖率测序数据的效率.

结论:

  • PMAT提供了一种高效准确的植物线粒体基因组组装方法.
  • 该工具包允许使用最小的测序数据进行高质量的线粒基因组组装.
  • PMAT将加速植物进化变异和育种应用的研究.