概括
天生的B1细胞及其天然抗体 (NAbs) 对于由损伤相关分子模式 (DAMPs) 触发的过敏呼吸道疾病 (AAD) 的发病至关重要. 然而,它们不会影响病原体相关分子模式 (PAMP) 驱动的AD.
科学领域:
- 免疫学 免疫学 免疫学
- 过敏研究 研究过敏
- 具有天生的免疫力.
背景情况:
- 过敏性呼吸道疾病 (AAD) 涉及2型炎症,由CD4 Th2细胞,巨细胞和基细胞驱动.
- 天生的B1细胞及其天然抗体 (NAbs) 在ADD病变发生过程中的作用尚不清楚.
- B1细胞检测损伤和病原体相关的分子模式 (DAMP和PAMP),影响免疫反应.
研究的目的:
- 研究B1细胞和NAbs在AAD不同小鼠模型中的作用.
- 为了确定DAMPs或PAMPs是否在AAD中差异性参与B1细胞介导免疫.
- 阐明NAbs影响2型免疫原始化的机制.
主要方法:
- 使用了缺乏B细胞,NAbs或所有分泌的抗体的转基因小鼠模型.
- 采用了由DAMPs (,尿酸) 或PAMPs (室内灰尘,Aspergillus) 触发的AAD的小鼠模型.
- 在各种淘汰赛和野生类型小鼠菌株中评估了eosinophilia和Th2细胞原始化.
主要成果:
- 缺乏B细胞或NAbs的小鼠在DAMP诱导的AAD模型中显著减少了eosinophilia和Th2原始化.
- 在敏感化期间在B细胞缺乏的小鼠中恢复B1细胞或NAbs,恢复了eosinophilia.
- 对过敏原的PAMP依赖的2型免疫反应不受NAbs缺失的影响.
结论:
- 由B1细胞产生的天然抗体在AAD中驱动DAMP诱导的2型免疫力中发挥着关键的,选择性的作用.
- 在DAMP驱动的过敏中,NAbs对于授权抗原呈现细胞启动2型免疫反应至关重要.
- B1细胞和NAbs对AAD的贡献是特定于启动触发器的,区分DAMP和PAMP.
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