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Updated: Jul 1, 2025

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
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Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

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在大肠杆菌中通过MBP标记表达检测人体蛋白质的histidine polyphosphate修饰的协议

Kirsten Lehotsky1, Nolan Neville1, Zongchao Jia1

  • 1Department of Biomedical and Molecular Sciences, Queen's University, Kingston K7L 3N6, Canada.

STAR protocols
|March 12, 2024
PubMed
概括

这项研究引入了一种检测人类蛋白质中胺聚酸盐修饰 (HPM) 的方法. 该协议使用大肠杆菌表达和凝电泳来识别这种独特的蛋白质修饰.

关键词:
分子生物学分子生物学蛋白质生物化学 蛋白质生物化学蛋白质的表达和净化过程

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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