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一个简单的H (C/C) 过实验,用于量化和追踪复杂的环境和生物样本中的同位素丰富

Katrina Steiner¹, Wolfgang Bermel², Ronald Soong¹

¹Environmental NMR Center, University of Toronto, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.

Journal of magnetic resonance (San Diego, Calif. : 1997)

|March 12, 2024

概括

一种新的核磁共振 (NMR) 方法使用"in-phase/opposite-phase"编码来同时检测和区分12C和13C结合的质子. 这种技术为复杂的生物和环境标记物研究提供了高灵敏度.

科学领域:

生物化学生物化学
环境科学环境科学
医学研究医学研究

背景情况:

异质的生物和环境样本在核磁共振 (NMR) 光谱中存在挑战,原因是光谱重叠和低信号.
传统的13C追踪方法通常依赖于卫星比率,而在复杂的混合物中,这种比率可能是有限的.

研究的目的:

引入一种新的1D 1H-12C/13C核磁共振方法,用于同时检测和区分与12C和13C结合的质子.
为了实现复杂样品的定量和定性分析,提高灵敏度和减少光谱重叠.

主要方法:

使用1D 1H-12C/13C NMR序列的开发使用1D 1H-12C/13C NMR序列的开发.
在相位/相反相位.
编码. 编码. 在编码.
分离12C和13C结合的质子子谱.
使用参考频谱,对1H-12C和1H-13C对之间的差异性放松率进行补偿.

主要成果:

该方法成功地为12C和13C结合的质子创建了单独的子光谱,保持了几乎完全的1H灵敏度.
用葡萄糖混合物和藻类细胞进行的概念验证研究产生了12C/13C比率,与预期值的偏差小于1%.
在大方中对13C丰富的量化显示,饮食中的13C脂质与固有的12C脂质的同化.

结论:

开发的NMR方法为复杂样品中的13C追踪提供了一种敏感,多功能和简单的方法.
这种技术非常适合用于各种科学学科的流量学和其他追踪器研究.
该方法准确量化同位素丰富,并揭示代谢途径和同化过程.

关键词:

碳封存是一种碳封存方式.碳追踪是指碳的追踪.一个相位逆转的相位逆转.量化同位素歧视量化同位素歧视

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