胆酸的多样性被低估了
Ipsita Mohanty1, Helena Mannochio-Russo1, Joshua V Schweer2
1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, USA.
Cell
|March 12, 2024
概括
研究人员使用新的MS/MS光谱库发现了许多以前未知的胆酸修饰. 这些修饰,包括多胺胆酸盐,在人类中发现,并随饮食而改变,影响健康和疾病研究.
科学领域:
- 代谢学
- 生物化学
- 微生物新陈代谢
背景情况:
- 宿主和微生物代谢对胆汁酸的改变尚不完全理解.
- 对于研究它们在健康和疾病中的作用至关重要.
研究的目的:
- 创建一个资源来识别新的胆酸修饰.
- 研究特定胆汁酸变化的流行程度和饮食影响.
主要方法:
- 过了 12 亿个公开的双重质谱 (MS/MS) 对胆酸选择性离子图案的光谱.
- 开发了一种以胆汁酸为中心的MS/MS光谱库.
- 利用图书馆识别新型代谢物,包括多胺胆固醇.
主要成果:
- 在物种和微生物培养中发现了数千种以前未知的胆酸变异.
- 发现的多胺胆酸盐在食肉动物中普遍存在,在人类中也存在.
- 在饮食变化后观察到的人类聚胺胆酸盐水平的变化.
结论:
- 胆汁酸的变化明显超过以前的数据.
- 可以利用公开的代谢学数据来发现新代谢物.
- 开发的MS/MS图书馆将促进胆酸在健康和疾病中的功能研究.
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