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来自Xanthoria elegans的李氏菌含多糖和其有效保护LX-2细胞免受H₂O₂诱导的氧化损伤的能力

Zheng Zhou¹, Guoqiang Li¹, Liang Gao¹

¹Qinghai Provincial Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining 810001, China; University of Chinese Academy of Sciences, Beijing 100049, China.

International journal of biological macromolecules

|March 12, 2024

概括

来自 Xanthoria elegans 的多糖化合物对肝脏健康有很大的前景. 在细胞模型中,XEP-70微分体显示出显著的抗氧化和肝脏保护作用,这表明了潜在的治疗应用.

科学领域:

植物化学植物化学
药理学药理学是指药理学的学科.
肝病学肝病学是一种肝病学.

背景情况:

香 (Xanthoria elegans) 是一种在传统中医中使用的,以治疗肝脏疾病而闻名.
多糖是X. elegans中关键的生物活性化合物,但它们的肝保护作用在很大程度上仍未得到研究.

研究的目的:

评估来自X. elegans的多糖分的肝脏保护潜力.
为了确定活性多糖分的化学成分.
为了研究抗氧化性肝损伤的作用机制.

主要方法:

从X. elegans.中提取和净化多糖分 (XEP-50,XEP-70,XEP-90) 的部分.
在体外抗氧化剂测试 (自由基清除,降低功率).
在LX-2细胞中使用过氧化诱导的氧化损伤模型评估肝脏保护作用.
活性多糖分的结构阐明.
对Nrf2/Keap1/ARE信号通路的分析.

主要成果:

与XEP-50和XEP-90相比,XEP-70表现出更高的抗氧化活性.
XEP-70是一种含有点素的异多聚糖,结构复杂,包括葡萄糖,银河糖,曼诺糖和阿拉伯银河糖的单位.
XEP-70有效地保护LX-2细胞免受过氧化引起的氧化损伤.
保护机制包括通过激活Nrf2/Keap1/ARE信号通路来增强细胞的抗氧化能力.

结论:

来自Xanthoria elegans的多糖分数XEP-70具有显著的抗氧化和肝保护性质.
XEP-70显示了保护肝脏恒星细胞免受氧化应激的潜力.
进一步研究XEP-70作为治疗肝病的治疗剂是有必要的.

关键词:

具有抗氧化活性的抗氧化剂.肝脏的星状细胞是什么李氏是一种.质素 (Pectin) 是一种可怕的成分.桑托里亚优雅的植物

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