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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 1, 2025

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
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Published on: June 26, 2019

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一个单细胞基因组策略用于替代转录开始地点的识别.

Yanling Peng1, Qitong Huang1,2, Danli Liu1

  • 1Animal Functional Genomics Group, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

Biotechnology journal
|March 12, 2024
PubMed
概括

识别替代转录起点 (TSSs) 对于基因调节至关重要. 这项研究引入了一种新的单细胞基因组技术,以精确地绘制TSS并检测细胞异质性,揭示了比以前知道的更多的替代TSS.

关键词:
分析生物技术分析生物技术生物信息学是一种生物信息学.

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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3' End Sequencing Library Preparation with A-seq2
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Last Updated: Jul 1, 2025

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 替代转录起点 (TSS) 的使用对于哺乳动物的基因转录调节至关重要.
  • 准确的全基因组识别替代TSS是一个重大挑战.

研究的目的:

  • 开发和应用一种新的单细胞基因组技术,用于替代的TSS注释.
  • 使用替代TSS配置文件检测细胞异质性.
  • 调查IFN-β刺激小鼠胚胎纤维细胞 (MEFs) 中替代性TSS的流行情况.

主要方法:

  • 使用Fluidigm C1系统进行单细胞捕获.
  • 雇佣了SMARTercDNA合成套件,用于全长的cDNA恢复.
  • 在基因组分析中应用双化寡核酸系统用于TSS丰富.

主要成果:

  • 成功地区分了两个IFN-β刺激的MEF群体.
  • 确定81%的表达基因具有多个TSS,超过了之前的估计 (CAGE:58%,经验:54%).
  • 证明了该技术在检测替代TSS方面的高度灵敏性.

结论:

  • 替代的TSS比以前理解的更为广泛.
  • 开发的单细胞基因组策略为TSS识别提供了前所未有的灵敏度.
  • 这种方法为未来关于替代TSSs生物意义的研究提供了一个强大的工具.