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使用NADH异构性来研究线粒体晶体对齐的研究

Holly E Smith¹, Alasdair M Mackenzie¹, Chloe Seddon^2,3

¹UKRI, STFC, Central Laser Facility, Rutherford Appleton Laboratory, Oxfordshire, OX11 0QX, UK.

Scientific reports

|March 13, 2024

概括

这项研究表明,线粒体表现出有序结构和高定向性质,通过减少的尼古丁胺胺氨基二核酸 (NADH) 的光观察到. 这些发现表明生物过程中潜在的量子效应.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
量子生物学量子生物学

背景情况:

生命的过程涉及离子流和光敏感分子.
线粒体,关键的真核细胞器官,适应形态的恒常状态.
在活细胞中研究未染色的in vivo纳米结构具有挑战性.

研究的目的:

为了研究线粒体的in vivo纳米结构和动态.
为了探索降低尼古丁胺氨酸二核酸 (NADH) 作为光探针的作用.
评估生物系统中的潜在量子效应.

主要方法:

利用NADH (340-360nm和730nm) 的一光子和两光子吸收光谱.
采用光终身成像和异性变异谱学.
在溶液,蛋白质/糖醇环境和活体MCF-7细胞中进行了NADH光成像.

主要成果:

在水溶液中的NADH显示显著的异构性 (~0.20),高于光素或.
在蛋白质 (0.30) 和糖醇 (0.42) 环境中,NADH异构性增加,表明有序结构.
对MCF-7细胞的双光子成像显示出强烈的NADH异构 (0.25-0.45),表明线粒体结构对齐.

结论:

线粒体表现出有序的,异型结构,可通过NADH光观察.
在紫外线激发下NADH的定向性质可能为生物学中的量子现象提供了见解.
进一步的研究可以探索电子和光子级联,自旋效应以及生物系统中的量子道.

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