维龙内拉卡利斯 (Veillonella faecalis sp.) 是一种植物. nov.,一种从婴儿便中分离出来的产生酸的细菌
Haneol Yang1,2, Hana Jo1, Seung Hyun Kim1
1Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-Gil, Jeongeup-Si, Jeollabuk-Do, 56212, Republic of Korea.
Antonie van Leeuwenhoek
|March 13, 2024
概括
一种新的酸产生细菌,Veillonella faecalis sp. 11月,从一个婴儿身上鉴定出来.
科学领域:
- 微生物学和微生物分类学
- 肠道微生物组研究研究
- 细菌基因组学 细菌基因组学
背景情况:
- 维龙科家族包括参与各种代谢过程的无氧菌.
- 了解人类肠道微生物群的多样性对于健康和疾病研究至关重要.
- 识别新的细菌物种有助于全面了解微生物生态系统.
研究的目的:
- 描述和分类从婴儿便样本中新分离的无氧性,阴性菌.
- 确定该分离物与Veillonella属内的已知物种的遗传学和基因组相关性.
- 根据全面的多相分类学分析,提出一种新的物种名称.
主要方法:
- 从韩国婴儿便样本中分离和培养无氧细菌.
- 16S rRNA基因测序用于遗传学分析.
- 平均核酸标识 (ANI) 计算用于基因组比较.
- 现型表征包括形态学,格拉姆染色,催化酶试验和发酵最终产品分析.
- 细胞脂肪酸组成的化学分类学分析.
主要成果:
- 一种新型细菌菌株Ds1651T被分离出来,并被描述为严格无氧的,阴性菌.
- 遗传学分析显示,16S rRNA基因序列与Veillonella nakazawae (99.86%) 的相似性最高.
- 基因组分析显示,平均核酸标识 (ANI) 值低于与相关的Veillonella物种的物种划分门.
- 该菌株作为主要发酵最终产品产生了酸和酸,并具有38.58%的G + C含量.
- 主要的细胞脂肪酸包括C17:1 ω8c和/或C17:2,和C13:0.
结论:
- 综合的遗传学,基因组学,表型学和化学分类学数据支持Ds1651T被归类为一种新物种.
- 这种新物种的建议名称是Veillonella faecalis sp. 新的,与类型菌株Ds1651T (=KCTC 25477T=GDMCC 1.3707T) 相关.
- 这一发现扩大了Veillonella属在人类肠道微生物群中的已知多样性.
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