在酵母中共享多态的古代和最近的起源
Nicolò Tellini1, Matteo De Chiara1, Simone Mozzachiodi1
1CNRS, INSERM, IRCAN, Côte d'Azur University, Nice, France.
Nature ecology & evolution
|March 13, 2024
概括
在Saccharomyces cerevisiae和Saccharomyces paradoxus之间共享的遗传材料揭示了最近的杂交事件和基因流动. 这项研究绘制了进化DNA块的地图,揭示了功能基因变异,这些变异增强了酵母中的抗真菌药物耐药性.
科学领域:
- 进化生物学是进化的生物学.
- 遗传学 是一个遗传学.
- 微生物学 微生物学
背景情况:
- 共同的遗传多态性可能来自于祖先的变异或最近的基因流动在种群和物种之间.
- 了解这些过程是追踪进化历史和人口动态的关键.
研究的目的:
- 在Saccharomyces cerevisiae和Saccharomyces paradoxus之间绘制共享的多态图.
- 调查这些物种之间的分歧和次要接触的历史.
- 为了确定进化遗传物质的功能后果.
主要方法:
- 在1673个测序的S. cerevisiae分离物中利用了单核酸诊断标记物的密集地图.
- 通过使用高分辨率的遗传标记,从S. paradoxus中目录了进化序列块.
- 经过实验设计的进化基因对 (PAD1-FDC1) 来评估功能影响.
主要成果:
- 确定了3,852个从S. paradoxus到S. cerevisiae的内进序列块,主要是最近的和类特定的.
- 在中国的S. cerevisiae血统中观察到枯竭的内向,过多的祖先多态度表明不完整的血统分类.
- 在非中国血统中检测到主要的杂交事件和重叠的进化块,表明共享的历史或融合的进化.
- 证明了内进的PAD1-FDC1基因对会增加抗真菌药物的耐药性.
结论:
- 这项研究重建了S. cerevisiae和S. paradoxus的进化轨迹,突出了复杂的分歧和基因流动模式.
- 确定了具有重大功能影响的特定内进基因元素,例如增强药物耐药性.
- 提供了对塑造密切相关物种遗传变异的机制的见解.
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