脂质平衡对于丝虫,Bombyx mori的 oogenesis 和胚胎生成至关重要
Fangying Yang1, Xiaoyan Xu2, Bo Hu3,4
1Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
Cellular and molecular life sciences : CMLS
|March 13, 2024
概括
脂质平衡对于昆虫的繁殖至关重要. 这项研究揭示了布鲁默脂酶 (BmBmm) 和激素敏感脂酶 (BmHsl) 以及BmSrebp如何协调脂质代谢,使丝虫的生殖和胚胎发育取得成功.
科学领域:
- 昆虫的生殖生物学 昆虫的生殖生物学
- 脂质新陈代谢和恒常状态.
- 在繁殖过程中的能量平衡.
背景情况:
- 生殖从根本上与能量平衡有关,需要动态的脂质合成和动员.
- 在昆虫的繁殖发育过程中,精确协调脂质稳态仍然不太清楚.
- 丝虫 (Bombyx mori) 作为研究生殖过程中的能量代谢的模型.
研究的目的:
- 阐明Bombyx mori.中的能量代谢和繁殖之间的关系.
- 调查两个主要的脂质激酶,布鲁默 (BmBmm) 和激素敏感脂酶 (BmHsl),以及固醇调节元素结合蛋白 (BmSrebp) 在生殖过程中的作用.
- 了解脂质稳态如何支持女性的生殖成功.
主要方法:
- 在CRISPR/Cas9中介突变分析中.
- 对BmBmm,BmHsl和BmSrebp的功能性调查
- 利皮多米学分析
- 对转录组进行分析.
主要成果:
- BmBmm对于女性的生育能力至关重要,调节三糖的储存并影响胚胎脂质组成.
- BmHsl主要控制胚胎发育的固醇代谢,而不是三糖代谢.
- 在脂肪体中,BmBmm缺乏会调节脂质合成基因,包括BmSrebp.
- BmSrebp被确定为卵细胞脂质积累的关键调节者,促进卵细胞生成并与BmBmm合作.
结论:
- 脂质平衡对于丝虫的雌性生殖成功至关重要.
- BmBmm和BmSrebp合作,以满足卵细胞生产的代谢需求.
- 澄清了BmBmm和BmHsl在调节生殖和发育期间脂质和固醇代谢中的不同作用.
相关概念视频
Oogenesis
63.7K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.7K
Hormonal Control of the Ovarian Cycle
496
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
496
Asymmetric Lipid Bilayer
7.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.2K
Ovarian Cycle
1.2K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
1.2K
Folliculogenesis
765
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
765
Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K


