常规授精和细胞内注射精子所产生的胚胎中的分泌体不同调节体外子宫内细胞
Ameya Jijo1, Itti Munshi2, Shubhashree Uppangala3
1Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Manipal Academy of Higher Education, Manipal, 576 104, India.
Reproductive sciences (Thousand Oaks, Calif.)
|March 13, 2024
概括
传统授精 (CI) 和细胞内注射精子 (ICSI) 胚胎分泌体对子宫内膜细胞的影响不同. CI胚胎增加整合蛋白表达,而ICSI胚胎提高MUCIN-1,这表明在辅助生殖中具有明显的植入潜力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学 细胞和分子生物学
- 辅助生殖技术 (ART) 是一种辅助生殖技术.
背景情况:
- 传统授精 (CI) 和细胞体内精子注射 (ICSI) 是标准的ART程序.
- 关于CI与ICSI胚胎的发育能力和植入潜力的数据仍然没有确定性.
- 胚胎分泌体可能会影响子宫内膜受体.
研究的目的:
- 研究CI和ICSI衍生的胚胎分泌体对人类子宫内膜上皮细胞的差异性影响 (石川).
- 分析对整合素 (αvβ3) 和MUCIN-1 (MUC-1) 表达的影响.
主要方法:
- 一项涉及23名ART患者的前性研究.
- 用来自CI和ICSI衍生的胚胎的秘密体对石川细胞进行治疗.
- 针对αvβ3整蛋白和MUC-1表达的免疫细胞化学分析.
主要成果:
- 与ICSI衍生的分泌体相比,CI衍生的胚胎分泌体在石川细胞中显著增加了αvβ3整合素表达.
- MUC-1转录水平没有显著差异.
- 与CI衍生的分泌体相比,ICSI衍生的胚胎分泌体显著增加了MUC-1表达.
结论:
- 来自CI和ICSI方法的胚胎分泌体在体外不同调节子宫内膜上皮细胞.
- 这些发现表明,CI和ICSI衍生的胚胎在影响植入子宫内膜环境的能力方面存在潜在的差异.
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