三级:有前途的种类可以改善健康
Salvatore De Caro1, Antonella Venezia1,2, Luigia Di Stasio1
1Institute of Food Science, National Research Council (ISA-CNR), 83100 Avellino, Italy.
Foods (Basel, Switzerland)
|March 13, 2024
概括
一种混合谷物Tritordeum显示出更健康食品的潜力. 它的品种Aucan和Bulel与 durum小麦相比,表现出较低的毒性和更好的肠道屏障完整性,这表明它们可能会减轻小麦面粉的毒性.
科学领域:
- 食品科学 食品科学 食品科学
- 植物育种 植物育种
- 细胞生物学 细胞生物学
背景情况:
- 特里托德 (Tritordeum) 是一种来自 durum wheat 和野生大麦的新型两类谷物.
- 它具有可取的感官,农学和技术特性,使其成为健康食品的候选者.
- 评估其生物效应对于食品开发至关重要.
研究的目的:
- 为了评估蛋白质消化的Tritordeum品种 (Bulel和Aucan) 对Caco-2细胞的生物学影响.
- 为了比较Tritordeum蛋白质消化剂与 durum小麦蛋白质消化剂的影响.
- 为了确定Tritordeum在减轻小麦面粉毒性的潜力.
主要方法:
- 从Tritordeum种类和 durum小麦中提取和消化使用胃/胰腺蛋白酶的蛋白质.
- 消化蛋白样本 (DPs) 应用于在跨井插件上培养的分化Caco-2细胞.
- 试验包括细胞活力,单层透性 (紧密结合完整性),F-actin微纤维组织和ER应力标志物.
主要成果:
- 坚硬小麦蛋白消化剂 (Provenzal-DPs) 迅速破坏了Caco-2细胞紧密结合屏障,并降低了细胞活力.
- 三级Aucan-DPs没有增加单层透性,而Bulel-DPs只显示了轻微的影响.
- 与Provenzal-DPs不同,Aucan-DPs和Bulel-DPs没有对Caco-2细胞活力或细胞骨架结构产生负面影响.
结论:
- 与 durum小麦相比,Tritordeum品种,特别是Aucan和Bulel,具有较低的诱导毒性和肠道屏障破坏的能力.
- 这些发现表明,Tritordeum具有可能有助于减轻与传统小麦面粉相关的不良影响的特性.
- 三角代表了一种有前途的替代谷物,可以开发出更安全,更健康的食品.
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