通过使用玉米液水解剂作为替代蛋白质源的Cordyceps Militaris改善了 Cordycepin的生产
Ying Chang1,2, Xiaolan Liu2, Yan Jiao2
1College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Foods (Basel, Switzerland)
|March 13, 2024
概括
玉米液水解剂 (CSLH) 在Cordyceps militaris.的水下培养物中显著提高了cordycepin的产量. 这种具有成本效益的替代源增强了菌根的生长和皮质的产量,提供了高效,低成本的生产.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 工业微生物学 工业微生物学
背景情况:
- Kordycepin 是一种有价值的生物活性化合物,由 Cordyceps militaris 生产.
- 对于Cordyceps militaris发酵来说,传统的源可能是昂贵的.
- 探索替代的,具有成本效益的源对于高效的皮生产至关重要.
研究的目的:
- 评估玉米液水解盐 (CSLH) 作为可替代的源,用于生产皮.
- 为了优化CSLH度,以增强菌体生长和cordycepin产量.
- 为了研究CSLH对Cordyceps militaris的代谢作用.
主要方法:
- 使用CSLH作为源的Cordyceps militaris的潜水培养.
- 测试CSLH的不同度,以确定最佳的诱导水平.
- 分析细胞生长,皮质蛋白生产和代谢特征 (糖,氨基酸,核酸).
主要成果:
- 在不同的C. militaris菌株中,CSLH显著增强了菌体生长和cordycepin生产.
- 最优的菌株C. militaris GDMCC5.270显示出对CSLH的优越利用.
- 1.5g/L的CSLH度产生了343.03mg/L的cordycepin,与对照组相比增加了4.83倍.
- CSLH改善了关键营养素的新陈代谢,支持增强的冠状腺素生物合成.
结论:
- 玉米液水解剂是Cordyceps militaris种植的经济有效和高效的替代源.
- CSLH促进细胞的生长,并显著增加皮质蛋白的积累.
- 这一发现为工业规模的皮生产提供了可持续和经济的方法.
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