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Updated: Jul 1, 2025

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解读子表达的表达:在生殖转录学中替代多基和lincRNAs的作用

Mingzheng Liu¹, Jiahao Chen¹, Chunlei Zhang¹

¹College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Animals : an open access journal from MDPI

|March 13, 2024

概括

雌性猪的雌性表达受基因和长跨基因非编码RNA (lincRNA) 差异表达和3'-未翻译区域 (3'-UTRs) 的替代多基化 (APA) 的调节. 这些分子机制为猪生殖生理学提供了新的见解.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
动物科学动物科学

背景情况:

雌激素的表达对于猪的生育能力和子大小至关重要.
了解的分子调节对于改善猪繁殖至关重要.

研究的目的:

阐明猪中雌性表达的调节机制.
在性期和性期期间分析和阴道组织中的差异性基因和lincRNA表达和替代多基化 (APA).

主要方法:

基因和lincRNA表达在和阴道组织中的基因和lincRNA表达的比较转录组分析.
在3个未翻译区域 (3-UTRs) 中对替代多基化 (APA) 事件的分析.
生物信息分析,包括途径丰富和miRNA结合部位预测.

主要成果:

观察到基因表达差异,阴道DEG富含神经和类固醇激素反应通路,而阴道DEG与代谢和VEGF信号通路有关.
鉴定了差异表达的lincRNAs (DELs),其中的目标基因在内分泌抵抗途径中得到丰富.
发现了显著的APA事件,包括3 -UTR延长,特别是在阴道中,经历APA的基因在亡或自途径中得到丰富.
延长的3 -UTRs显示了多个miRNA结合点和细胞质多化元素 (CPE) 调节特征,在特定基因如NFIX和ABHD2.2中验证了CPE.

结论:

替代多基化 (APA) 和lincRNAs在调节雌性猪的雌性表达方面发挥着重要作用.
这些发现为控制猪的机制提供了新的分子洞察力,可能会影响生殖效率.

关键词:

这是一个LncRNARNA.替代性的多基化.雌性的时间是 estrus.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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