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植物化学物质阿加西斯拉调节miR146a和miR155在涉及STAT3信号的激活微细胞中

Balbino Lino Dos Santos^1,2, Cleonice Creusa Dos Santos¹, Karina Costa da Silva¹

¹Laboratory of Neurochemistry and Cellular Biology, Institute of Health Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon S/N, Salvador 40231-300, BA, Brazil.

International journal of molecular sciences

|March 13, 2024

概括

阿加西斯弗拉降低了活性微质中的微RNA (miR) 和炎症媒介表达,为阿尔茨海默病 (AD) 和神经炎症治疗提供了潜力.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

微RNAs (miRs) 调节质细胞中的基因表达,并与阿尔茨海默氏症 (AD) 等神经退行性疾病有关.
激活的微质细胞有助于神经炎症,这是AD病变发生的关键因素.

研究的目的:

为了研究阿加西斯弗拉对微质细胞的抗炎作用.
为了确定是否agathisflavone调节了AD相关的激活微质中的microRNA表达.

主要方法:

人类微质细胞 (C20) 通过使用粉样β (Aβ) 或脂多糖 (LPS) 激活.
细胞接受了阿加西斯拉治疗,并分析了特定miRs (miR-146a,miR-155) 和炎症调解剂 (IL1-β,IL-6,NOS2,p-STAT3) 的表达水平.

主要成果:

Aβ和LPS诱导了微质中的炎症状态,增加了miR-146a,miR-155,IL1-β,IL-6和NOS2的表达.
阿加西斯拉治疗显著降低了Aβ或LPS诱导的miR-146a和miR-155表达.
阿加西斯弗拉还降低了炎症媒介IL1-β,IL-6和NOS2的表达,并降低了Aβ刺激细胞中的p-STAT3水平.

结论:

阿加西斯拉在由Aβ和LPS激活的微质中表现出抗炎性质.
微RNA在神经炎症和AD模型中起到作用,在agathisflavone的抗炎机制中发挥作用.

关键词:

在STAT3中,我们可以使用STAT3.阿加斯是斯拉的一种.这是一种抗炎药物.人类微质细胞这就是miRNAs.

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