联合GWAS,链接映射和转录组分析,揭示玉米植物架构相关特征的遗传基础
Xuefeng Lu1,2, Pengfei Liu1, Liang Tu1
1Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.
International journal of molecular sciences
|March 13, 2024
概括
这项研究确定了玉米植物结构的120个定量特征核酸 (QTNs) 和32个定量特征位点 (QTLs),包括植物高度,耳高度和叶数. 这些发现为改善玉米育种策略提供了宝贵的遗传资源.
科学领域:
- 植物遗传学 植物遗传学
- 玉米育种 玉米育种
- 农业科学 农业科学
背景情况:
- 玉米植物结构,包括植物高度 (PH),耳高度 (EH) 和叶数 (LN) 等特征,对于产量至关重要.
- 通过遗传资源改善植物结构是提高玉米植物密度和产量的关键.
研究的目的:
- 识别与玉米植物结构特征相关的定量特征核酸 (QTNs) 和定量特征位点 (QTLs).
- 通过生物育种为改善玉米植物结构提供遗传资源.
主要方法:
- 使用MaiseSNP50芯片对226条内生系和150条家族系进行基因型化,并按序列 (GBS) 进行基因型化.
- 在三个不同的环境中进行表型化.
- 使用MLM的全基因组关联研究 (GWAS) 和使用ICIM模型的链接映射.
主要成果:
- 确定了120个与植物架构特征 (PH,EH,LN) 相关的QTN和32个QTL.
- 在Zhangye环境中发现了叶数的主导QTL (qLN7-2).
- 在整个环境中发现了6个通常与PH,EH和LN相关的QTNs.
- 确定了参与植物结构调节和男性不育相互作用的候选基因 (Zm00001d021574和Zm00001d044730).
结论:
- 该研究为玉米植物架构提供了一套全面的QTN和QTL.
- 候选基因分析表明,自和男性不育途径在调节这些特征中的作用.
- 这些发现对未来的玉米育种计划有价值,旨在改善植物结构和产量.
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