在磁性纳米颗粒上对酶的顺序联合固定,以有效地生产l-烯酸
Sanjay K S Patel1, Rahul K Gupta1, Karthikeyan K Karuppanan1
1Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
International journal of molecular sciences
|March 13, 2024
概括
在磁性纳米颗粒上对l-arabinitol 4-脱酶 (LAD) 和尼古丁胺氨酸二核酸氧化酶 (Nox) 的顺序联合固定增强了l-xylulose的产生. 与单个或混合酶固定相比,这种策略提供了卓越的稳定性和可重复使用性.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 在生物技术中的纳米技术.
- 绿色化学 绿色化学
背景情况:
- 辅因子再生对于高效的生物转化过程至关重要.
- 多酶系统提供了增强的催化效率.
- 磁纳米颗粒为酶固定提供了一个多功能平台.
研究的目的:
- 在磁纳米粒子上研究和比较LAD和Nox的单个,混合和顺序联合固定.
- 使用固定酶增强l-arabinitol转化为l-xylulose的作用.
- 评估固定酶系统的稳定性,可重复使用性和活性.
主要方法:
- 在磁性纳米粒子上对纯化的l-arabinitol 4-脱酶 (LAD) 和尼古丁胺胺氨基二核酸氧化酶 (Nox) 进行固定.
- 采用个人,混合和连续的联合固定化策略.
- 酶负荷,活性,pH/温度概况和稳定性的表征.
主要成果:
- 个体固定产生了较高的固定和相对活动 (91.4%和98.8%).
- 相比于混合联合固定,顺序联合固定实现了更高的酶负载 (122 mg/g) 和更高的转化效率.
- 顺序联合固定酶表现出增强的pH值,温度和储存稳定性,以及高的可重复使用性.
结论:
- 在磁性纳米颗粒上对LAD和Nox的顺序联合固定是生产l-烯酸的高度有效策略.
- 这种方法在转换产量,稳定性和可重复使用性方面超过了个人和混合联合固定.
- 这项研究介绍了LAD和Nox在磁性纳米粒子上进行混合或顺序联合固定的第一份报告,以提高生物转化.
关键词:
作为辅助因素的再生再生.联合不动化联合不动化一个l-arabinitol 4-脱酶.一个l-xylulose的类型.尼古丁胺胺腺氨酸二核酸氧化酶是什么可重复使用性可重复使用性.稳定的稳定性 稳定的稳定性更多相关视频
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