人类巨细胞激活旁观者中性粒细胞的新陈代谢和效应器功能,当受到Mycobacterium结核病的挑战时
Dearbhla M Murphy1, Anastasija Walsh1, Laura Stein1
1Department of Clinical Medicine, School of Medicine, Trinity Translational Medicine Institute (TTMI), Trinity Centre for Health Sciences, St. James's Hospital, Trinity College Dublin, The University of Dublin, Dublin 8, D08 W9RT Dublin, Ireland.
International journal of molecular sciences
|March 13, 2024
概括
巨细胞影响中性粒细胞的功能,增强它们的炎症和代谢活动,同时减少迁移. 这种巨细胞-中性粒细胞轴会影响诸如肺炎和结核病等疾病.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 中性粒细胞对于病原体清除至关重要,但在肺炎和结核病 (TB) 等感染中可能导致过度炎症.
- 免疫细胞相互作用对肺内的中性粒细胞功能的影响尚未完全理解.
研究的目的:
- 通过检查受刺激的人类单细胞衍生巨细胞 (hMDMs) 的条件介质对中性粒细胞功能的影响来研究巨细胞-中性粒细胞轴.
- 了解巨细胞衍生因素如何调节中性粒细胞活化,新陈代谢和迁移.
主要方法:
- 主要的人类单细胞衍生的巨细胞 (hMDMs) 被LPS或Mycobacterium tuberculosis刺激.
- 来自刺激的hMDMs的条件介质 (MΦ-CM) 用于治疗中性粒细胞.
- 评估了中性粒细胞激活,氧化和糖解代谢,ROS产生,NET形成,细胞因子释放 (CXCL8,IL-13,IL-6) 和迁移潜力.
主要成果:
- 受到刺激的hMDMs的条件介质显著增强了中性粒细胞的激活.
- 暴露于MΦ-CM的中性粒细胞显示氧化和糖解代谢升高,ROS产量增加,NET形成升高.
- 暴露于MΦ-CM也导致CXCL8,IL-13和IL-6的释放增加,但减少了中性粒细胞的迁移能力.
结论:
- 巨细胞衍生因素激活中性粒细胞,增强它们的代谢和效应器功能,同时损害它们的迁移.
- 巨细胞-中性粒细胞轴的这种调节有助于感染部位的炎症.
- 这些发现可能会为肺炎和活性结核病等炎症性疾病的免疫疗法提供信息.
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