相关实验视频

Updated: Jul 1, 2025

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

在神经母细胞瘤中,BAP31通过加列-3上调促进血管生成

Mwichie Namusamba¹, Yufei Wu¹, Jiaying Yang¹

¹College of Life Science and Health, Northeastern University, 195 Chuangxin Road, Hunnan District, Shenyang 110819, China.

International journal of molecular sciences

|March 13, 2024

概括

B细胞受体关联蛋白31 (BAP31) 通过增加血管内皮生长因子A (VEGFA) 和Galectin-3 (GAL-3) 来促进神经母细胞瘤 (NB) 血管生成. 这一发现为儿童固体瘤提供了新的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

神经母细胞瘤 (NB) 是一种高度血管化的儿童固体瘤.
了解NB血管生成是有效治疗的关键.
B细胞受体关联蛋白31 (BAP31) 在血管生成中的作用尚不清楚.

研究的目的:

调查BAP31在NB中调节亲血管性因素中的作用.
确定BAP31是否影响血管生成相关的信号通路.
在NB中探索BAP31作为潜在的治疗点.

主要方法:

使用SH-SY5Y NB细胞系进行实验.
采用蛋白质过度表达,敲击和抗体阻断技术.
量化蛋白质水平和评估内皮细胞迁移和管形成.

主要成果:

BAP31的过度表达增加了VEGFA和GAL-3的水平.
来自BAP31过度表达细胞的条件介质促进了内皮细胞迁移和管形成.
通过对HIF-1α和GAL-3的调节,BAP31增强了毛细管的形成,通过对Jagged 1和VEGFR2的上调调节.

结论:

BAP31通过调节GAL-3和VEGF信号通路来促进NB血管生成.
BAP31通过亲血管生成机制影响瘤微环境.
这项研究揭示了BAP31在神经母细胞瘤中的新型亲血管性作用.

关键词:

BAP31 是一个很好的方法.盖莱克-3 是一种在VEGFA中,VEGFA是VEGFA.血管新生是因为血管新生.癌症癌症癌症癌症癌症有条件的媒体.神经母细胞瘤的神经母细胞瘤

更多相关视频

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...