Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Modelling human skin morphology and simulating transdermal transport of 50 chemicals.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

Constraining safe and unsafe overshoots in saddle-node bifurcations.

Chaos (Woodbury, N.Y.)·2025
Same author

Intracellular "In Silico Microscopes"-Comprehensive 3D Spatio-Temporal Virus Replication Model Simulations.

Viruses·2024
Same author

Transdermal and lateral effective diffusivities for drug transport in stratum corneum from a microscopic anisotropic diffusion model.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2023
Same author

Influence of T-Bar on Calcium Concentration Impacting Release Probability.

Frontiers in computational neuroscience·2022
Same author

Dose-response functions and surrogate models for exploring social contagion in the Copenhagen Networks Study.

The European physical journal. Special topics·2021

相关实验视频

Updated: Jul 1, 2025

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

12.9K

对于空间时间解析的病毒复制动态的表面扩散参数的有效估计.

Markus M Knodel1, Gabriel Wittum2, Jürgen Vollmer3

  • 1Simulation in Technology, TechSim, 75248 Ölbronn-Dürrn, Germany.

International journal of molecular sciences
|March 13, 2024
PubMed
概括

一个新的缩放分析简化了估计C型肝炎病毒非结构性蛋白 (NSPs) 在内分泌网膜 (ER) 上的扩散系数. 这种方法使用几何比率,提高对潜在新疗法病毒复制的理解.

关键词:
(表面) 部分微分方程.3D空间时间解析的数学模型.几何学的影响力型肝炎病毒 (HCV) 病毒参数估计的参数估计.物理病毒学 物理病毒学现实的几何形状,现实的几何形状.扩展分析分析扩展分析病毒动态 病毒动态在主机内部的病毒建模.

更多相关视频

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

8.7K
Estimating Virus Production Rates in Aquatic Systems
10:49

Estimating Virus Production Rates in Aquatic Systems

Published on: September 22, 2010

12.7K

相关实验视频

Last Updated: Jul 1, 2025

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

12.9K
Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

8.7K
Estimating Virus Production Rates in Aquatic Systems
10:49

Estimating Virus Production Rates in Aquatic Systems

Published on: September 22, 2010

12.7K

科学领域:

  • 生物物理学的生物物理.
  • 病毒学 病毒学
  • 细胞生物学 细胞生物学

背景情况:

  • 病毒性疾病的先进治疗需要了解病毒与宿主之间的相互作用.
  • 肝炎C病毒 (HCV) 非结构性蛋白 (NSPs),如NS5A,对于复制和对内质网膜 (ER) 的定至关重要.
  • 之前的研究使用光漂白后光恢复 (FRAP) 和表面部分微分方程 (sufPDEs) 来分析NSP扩散.

研究的目的:

  • 开发一种简化的缩放分析,用于估计ER膜上NSP的扩散系数.
  • 通过数值解决方案和实验数据来验证这种新的分析方法.

主要方法:

  • 对SufPDEs的缩放分析,这些SufPDEs控制了NSP在ER上的传播.
  • 在平面和3D ER几何上进行sufPDEs的数值模拟.
  • 缩放分析估计与数值解决方案和实验FRAP数据的比较.

主要成果:

  • 一种新的方法估计了基于膜表面积与FRAP区域的轮长度的比率的扩散系数.
  • 缩放分析提供了与从广泛的数值模拟中得出的估计值相似的估计值.
  • 该方法成功地应用于现有的实验FRAP时间序列数据.

结论:

  • 开发的缩放分析为确定NSP扩散系数提供了更有效的方法.
  • 这种对ER的NSP动态的生物物理洞察力可以为开发新型抗病毒策略提供信息.
  • 简化方法增强了对病毒复制机制的分析,并有助于疫情准备.