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利用Leishmania原型的树突细胞作为犬类免疫反应的潜在免疫调节器
Ana Valério-Bolas1, Mafalda Meunier1, Joana Palma-Marques1
1Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisbon, Portugal.
Cells
|March 13, 2024
概括
这项研究揭示了Leishmania寄生虫及其细胞外囊泡如何激活犬类树突细胞 (DC). L. infantum促进了促炎反应,而L. amazonensis则诱导了混合反应,影响了莱什曼病免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
背景情况:
- 树突细胞 (DCs) 是通过激活T细胞来弥合先天性和适应性免疫的关键.
- 遇到Leishmania寄生虫时DCs的激活机制在很大程度上仍然没有表征.
研究的目的:
- 在暴露于Leishmania infantum和Leishmania amazonensis寄生虫及其细胞外囊 (EVs) 时,研究犬类单细胞衍生的树突细胞 (moDCs) 的体外激活.
主要方法:
- 狗的moDCs暴露于L.infantum和L.amazonensis寄生虫及其EVs.
- 分析了类似收费受体4基因表达,核因子kB激活,细胞因子概况,MHC类I和II表达以及共刺激分子CD86.
- 评估化学激素CXCL16释放的情况.
主要成果:
- 婴儿上调的TLR4,NF-κB,促炎细胞因子,MHCII类,CD86和CXCL16,促进T细胞激活.
- 在moDCs中,L. amazonensis诱导了一种混合的促炎和抗炎细胞因子配置.
- EVs调节moDCs以表达MHC类I,共刺激分子和CXCL16,可能吸引细胞毒性CD8+T细胞.
结论:
- 莱什曼尼亚寄生虫及其EV不同地塑造了狗的DC激活通路.
- 了解Leishmania的DC激活对于开发新的Leishmaniasis控制策略至关重要.
关键词:
L. 婴儿的婴儿.莱什曼亚亚马逊人种 (Leishmania amazonensis) 是一个亚马逊人种.莱什曼尼亚细胞外囊泡.细胞因子 细胞因子狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗这是先天免疫力.主要的组织相容性复杂.模式识别受体的模式识别受体周围血液衍生的树突细胞 (moDCs).更多相关视频
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