相关实验视频

Updated: Jul 1, 2025

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay DRaCALA

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay DRaCALA

Published on: March 19, 2021

细菌AHL对植物周期性腺单酸盐含量的影响根据高性能液体染色学

Xuemeng Zhao^1,2, Wen Li², Xiliu Li²

¹School of Biological Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China.

Molecules (Basel, Switzerland)

|March 13, 2024

概括

这项研究建立了一种高性能液体染色学 (HPLC) 方法,用于在植物中量化循环腺单酸盐 (cAMP). 该方法显示,细菌的定数感应信号显著增加了Arabidopsis中的cAMP水平,影响了植物与细菌的相互作用.

科学领域:

植物科学植物科学
分子生物学分子生物学
生物化学生物化学

背景情况:

循环腺单酸盐 (cAMP) 在细胞信号通路中起到关键的第二信使作用.
cAMP调节各种生物过程,包括生物体的生长,发育和应激反应.

研究的目的:

开发和优化高性能液体染色学 (HPLC) 方法,用于植物组织中cAMP的定量确定.
为了研究植物cAMP水平的变化,以响应细菌的定量检测信号 (N-acyl homoserine lactones - AHLs).

主要方法:

优化HPLC检测参数,包括列类型,移动相组合,检测波长,列温度和流量.
使用甲醇和超声波处理从阿拉比多普西斯中确定cAMP的最佳提取条件.
在暴露于AHLs后,应用开发的HPLC方法来量化Arabidopsis叶和根中的cAMP.

主要成果:

已建立的最佳HPLC条件:Venusil MP C18柱,甲醇-水 (0.1%三酸) 移动相 (10:90 v:v),259nm检测,35°C柱温度,0.8mL/分钟流量.
实现了高方法精度 (标准的98.21%,样品的98.87%) 和回收率 (101.067%).
证明细菌的AHL信号显著提高了Arabidopsis树叶和根中的cAMP水平.

结论:

开发的HPLC方法为量化植物cAMP提供了可靠和精确的工具.
细菌的AHL信号通路可以调节植物的细胞内cAMP水平,这表明它在植物-细菌通信中发挥了作用.
这项研究对于了解植物-细菌相互作用机制和信号传导通路至关重要.

关键词:

乳的N-乙烯同类素素.循环的腺单酸盐.确定内容的确定.高性能液体色谱学高性能液体色谱学植物植物植物植物植物.

更多相关视频

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

相关概念视频

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...