基于时间分辨率光共振能量转移技术的免疫测试系统
Zhengping Xu1, Hong Zhou1, Li Li1
1Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Sensors (Basel, Switzerland)
|March 13, 2024
概括
开发了一种新的时间解析光共振能量转移 (TR-FRET) 免疫测试系统,用于对多种生物标志物的敏感和特定检测. 这种TR-FRET系统提高了灵敏度和特异性,同时降低了联合检测的成本.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 传统的免疫试验在特异性,敏感性和成本效益方面面临挑战.
- 对生物标志物的多重检测对于准确的疾病诊断和监测至关重要.
- 时间解析的光共振能量转移 (TR-FRET) 提供了一种有前途的方法来克服这些局限性.
研究的目的:
- 开发和验证一种使用TR-FRET技术的新型免疫测试系统.
- 为了提高试验的特异性和灵敏性,同时降低检测成本.
- 为了能够同时检测多个生物标记物,包括前素 (PCT),C反应蛋白 (CRP) 和中白素-6 (IL-6).
主要方法:
- 设计和制造了一种包含光闪光灯,光倍增管 (PMT) 和过器切换机制的仪器,用于交替输送器和接受器光测量.
- 采用TR-FRET原理,利用接受器探针的长寿命光,提高灵敏度.
- 使用PCT,CRP和IL-6测定在各种度上验证了仪器的性能.
主要成果:
- 开发的仪器在低噪音水平和最小的温度变化下表现出色.
- 在特定范围内的发光值和生物标志物检测中实现了高线性 (R2 > 0.999).
- 达到PCT (0.096 ng/mL),CRP (2.70 ng/mL) 和IL-6 (2.82 ng/mL) 的敏感量化极限.
结论:
- 开发的TR-FRET免疫测试系统是可行的和有效的,用于敏感的,特定的和经济高效的多重生物标志物检测.
- 仪器和技术解决方案为先进的免疫测试提供了一个强大的平台.
- 这项技术有可能改善临床环境中的诊断能力.
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