相关实验视频

Updated: May 2, 2026

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Published on: June 29, 2018

开发和评估SNP基因型对及其近亲类的基因组

Yoko Hiraoka¹, Sergio Pietro Ferrante¹, Guohong Albert Wu²

¹Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.

Plants (Basel, Switzerland)

|March 13, 2024

概括

新的基因造型阵列为分析类植物基因组提供了强大的工具,揭示了遗传多样性并帮助了育种工作. 这些高密度SNP阵列为类和相关物种提供准确,可重复的数据.

科学领域:

基因组学就是基因组学.
植物科学植物科学
农业生物技术农业生物技术

背景情况:

技术进步使得水果作物基因组的详细分析能够理解遗传多样性并改善育种.
全基因组单核酸多态化 (SNP) 基因型化阵列提供了具有成本效益的,高通量遗传分析.

研究的目的:

开发和验证及其近亲的高密度SNP基因造型阵列.
评估这些数组的准确性,可复制性和实用性,用于基因组研究和育种.

主要方法:

开发1.4M和58K SNP Axiom®类基因型阵列,使用41个不同加入的类变种发现面板.
选择针对基因区域的SNP,以准确地调用类和相关品种.
通过可重现性,多态性分类 (PolyHighResolution) 和与序列数据一致的数组性能验证.

主要成果:

对于开发的SNP数组,几乎100%的可重现性和高精度 (98%与序列数据一致).
绝大多数SNP被归类为PolyHighResolution (PHR),表明其质量很高.
使用阵列数据的家族遗传学分析与序列数据的分析密切相似,即使在微小的基因型定型错误 (3-5%) 中也显示出稳定性.

结论:

开发的Axiom® Citrus HD和58K SNP数组是果基因组学的可靠和准确的工具.
这些公开可用的数组将大大帮助研究的遗传多样性,关系和育种计划.
这些阵列为各种类和相关物种提供了出色的基因组覆盖和标记质量.

关键词:

类植物中的类植物.基因造型阵列的基因造型阵列是什么?胚胎细胞质的胚胎细胞质.植物育种植物育种单个核酸的多态性.

更多相关视频

Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...