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在Lagerstroemia indica中的MADS-Box家族基因及其在花朵发育中的参与

Zhongquan Qiao¹, Fuyuan Deng¹, Huijie Zeng¹

¹Hunan Provincial Key Laboratory of Forest Clonal Breeding, Hunan Academy of Forestry, Changsha 410004, China.

Plants (Basel, Switzerland)

|March 13, 2024

概括

这项研究探讨了木的非果实性特征.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
植物生理学植物生理学

背景情况:

MADS盒子转录因子是开花和花朵发育的关键调节者.
木种类"云"表现出一种独特的无果特征.
了解这种特性的遗传和荷尔蒙基础对于植物育种至关重要.

研究的目的:

为了阐明 *Lagerstroemia indica* "Xiang Yun" 的非结果性质背后的分子机制.
为了识别和表征MADS盒子基因参与L. indica的花朵发育.
调查MADS盒子基因表达,激素水平和非结果的表型之间的关系.

主要方法:

在L. indica*中对82个MADS盒基因进行全基因组识别和生物信息学分析.
定量实时PCR (qRT-PCR) 和转录组分析以评估基因表达.
内生激素水平测量和与基因表达的相关性分析.

主要成果:

大多数已识别的*LiMADS*基因是II型,具有丰富的cis作用元素.
大多数与花朵发育相关的*MADS*基因在"云"与"洪叶"相比表达明显较低.
"云"在花朵发育过程中表现出植物激素 (细胞因素和斯酸盐) 的水平变化.

结论:

植物激素通路可能会影响MADS盒基因表达,导致"云"的表达减少.
低MADS盒基因表达有助于异常的干膜和胚胎囊发育,导致非结果的表型.
这项研究提供了一个模型来理解"云"的非果实性特征,并为木的育种提供了洞察力.

关键词:

Lagerstroemia indica 是一个指标性疾病.这是一个MADS盒子.花的器官是花的器官.基因分析基因分析家庭分析不孕不育不孕不育不孕不育植物激素植物激素

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