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从肉中分离和分子表征多药耐药的大肠杆菌

Anugya Jaiswal¹, Aquib Khan¹, Akanksha Yogi¹

¹Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India.

|March 13, 2024

概括

抗生素耐药性基因正在从动物农场传播. 这项研究在肉肉中发现了多药耐药性大肠杆菌,突出了家禽产品的风险,并倡导减少抗生素的使用.

科学领域:

食品微生物学食品微生物学
抗微生物耐药性抗微生物耐药性
分子生物学分子生物学

背景情况:

动物农业中的抗生素有助于抗生素耐药基因 (ARG) 和细菌 (ARB) 的扩散.
从畜牧场传播抗生素耐药性到环境中的传播是一个日益严重的公共卫生问题.

研究的目的:

从肉肉中分离和分子识别大肠杆菌 (大肠杆菌).
为了确定这些大肠杆菌分离物的抗生素耐药性概况.

主要方法:

从印度普拉雅格拉吉的零售商店收集肉肉样本.
对大肠杆菌的隔离和属性特定的PCR鉴定.
对抗9种抗生素和特定耐药基因的抗生素耐药性的表型查 (光盘扩散) 和基因型分析 (PCR).

主要成果:

鉴定出154种大肠杆菌分离物,其中64.28%呈现多药性耐药性 (MDR).
观察到对四环素 (78%),西普洛素 (57.8%),三甲素 (54.00%) 和红素 (49.35%) 的高耐药率.
在MDR分离物中,tetA和blaTEM基因最为普遍 (分别为84.0%和82.0%).

结论:

禽类产品含有MDR大肠杆菌,对消费者构成重大风险.
A和blaTEM基因的高患病率强调了这些隔离品中耐药性的遗传基础.
这些发现支持迫切需要限制家禽养殖中的抗生素使用,以减轻耐药性的传播.

关键词:

抗生素耐药性抗生素耐药性抗生素耐药性基因是抗生素耐药性的基因.肉肉类肉肉类美国大肠杆菌 (E. coli).它具有多种药物耐药性.

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