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在喘小鼠模型中,抗生素的使用通过破坏肠道微生物群和肠道粘膜屏障来加剧喘
Cheng-Ling Xu1, Cui Wang1, Gao-Bin Li1
1College of Basic Medical Sciences, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, P.R. China.
Experimental and therapeutic medicine
|March 13, 2024
概括
抗生素破坏肠道微生物群和肠道屏障,使喘症状恶化. 这项研究揭示了抗生素使用如何影响小鼠的肠道健康和喘严重程度.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 肠道微生物群在人类健康和疾病,包括喘中发挥着至关重要的作用.
- 抗生素 (ABXs) 显著改变肠道微生物群的组成和丰富性.
- 了解ABX对肠道微生物群和肠道屏障的影响对于喘管理至关重要.
研究的目的:
- 在小鼠喘模型中研究抗生素给药对肠道微生物群和肠道粘膜屏障功能的时间依赖性影响.
- 为了阐明肠道微生物群在喘中的调节机制.
主要方法:
- 使用卵胺 (OVA) 建立了小鼠喘模型,并为不同持续时间 (1或2周) 服用抗生素尾酒.
- 分析了肺部和结肠炎症细胞透,暂停值,免疫球蛋白E (IgE),TGF-β和紧结蛋白 (TJP1,Claudin 1,Occludin) 的表达.
- 量化肠道细菌负载和确定的主导细菌种群.
主要成果:
- 抗生素治疗加剧了喘病理,增加了炎症细胞透和IgE/TGF-β水平,同时降低了紧结蛋白的调节.
- 肠道细菌负载因抗生素治疗的持续时间和顺序而显著改变.
- 拉克诺斯皮拉和Escherichia-Shigella属的丰度与IgE和紧结蛋白表达相关.
结论:
- 抗生素的使用会破坏肠道微生物群的多样性和丰富性,损害肠道屏障的完整性,并促进炎症.
- 这些变化对喘缓解和长期修复产生不利影响.
- 准肠道微生物群调节可能是喘潜在的治疗策略.
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