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在Synsepalum dulcificum中探索内植物的多样性,生物活性和代谢

Sisi Liu¹, Yage Hou¹, Kaixuan Zheng¹

¹Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, China.

Frontiers in microbiology

|March 13, 2024

概括

奇迹果植物 (Synsepalum dulcificum) 的内植物显示出高多样性和产生生物活性化合物的潜力. 许多分离物体表现出抗微生物,抗氧化剂和α-葡萄糖酶抑制活性,这表明在药物开发中的应用.

科学领域:

微生物学和生物技术
自然产品化学自然产品化学
药理学药理学是指药理学的学科.

背景情况:

Synsepalum dulcificum以其食用和药用特性而闻名,具有尚未探索的内生菌微生物资源.
内生菌是植物组织中存在的微生物,经常产生生物活性二次代谢产物.
了解S. dulcificum中的内细胞多样性和功能可以揭示治疗化合物的新来源.

研究的目的:

为了研究 Synsepalum dulcificum 的微生物多样性 (真菌和细菌).
分析内菌群体和植物代谢物之间的相关性.
评估分离的内细胞的生物活动 (抗微生物,抗氧化剂,α-葡萄糖酶抑制).

主要方法:

高通量测序 (ITS和16S rDNA) 用于内菌和细菌的鉴定.
对S. dulcificum组织的代谢分析.
孤立菌株的遗传学分析.
使用标准测定方法培养内菌并评估抗微生物,抗氧化剂和α-葡萄糖酶抑制活性.

主要成果:

在各种植物组织中识别了4,913种真菌和1,703种细菌的安普利康序列变异 (ASV).
观察到内菌群体组成和植物衍生代谢物之间存在显著的相关性.
88.6%的分离物显示出抗微生物活性,55.7%的抗氧化活性,85%的真菌显示出α-葡萄糖酶抑制活性.

结论:

Synsepalum dulcificum 含有高度多样化的内生菌微生物群.
这些内植物具有产生具有抗微生物,抗氧化剂和低血糖特性的生物活性代谢物的巨大潜力.
这些发现为从S. dulcificum endophytes开发新型抗生素,抗氧化剂和低血糖剂提供了基础.

关键词:

它们中的一个是Synsepalum dulcificum.生物活动是生物活动.多样性的多样性多样性的多样性它们是内植物 (endophytes).代谢生物组的代谢生物组微生物组是一个微生物组.

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