血统特异性CYP80扩张和基素化物多样性在早期分离的尤迪科特中
Zhoujie An1,2, Ranran Gao3, Shanshan Chen3
1Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|March 13, 2024
概括
在Menispermum dauricum中发生的全基因组重复事件揭示了基素化物 (BIA) 多样性的演变. 基因扩张和MdCYP80G10等酶的组织特异表达驱动了这种化学变异.
科学领域:
- 植物基因组学 植物基因组学
- 进化生物学是进化的生物学.
- 生物化学 生物化学
背景情况:
- 科植物种类产生的各种化素类化合物 (BIA).
- 在BIA化学多样性背后的进化机制仍然不清楚.
- 早期分离的花提供了关于植物进化的见解.
研究的目的:
- 为了研究Menispermaceae中BIA多样性的基因组基础.
- 在Menispermum dauricum中重建BIA生物合成途径.
- 了解基因复制和表达在BIA进化中的作用.
主要方法:
- 染色体层次的基因组组合的Menispermum乳液.
- 转录组和代谢组分析.
- 酶的表征,包括新型细胞P450单氧化酶 (CYP450s).
主要成果:
- 在M.dauricum中确定了两个全基因组复制 (WGD) 事件,与Ranunculales共享,并且特定于Menispermum.
- 重建了BIA生物合成途径,确定了五个关键酶 (一个诺科克劳林合成酶 (NCS) 和四个CYP450s).
- 发现了一种新的叶子特异性酶,MdCYP80G10,在BIA合成中催化立体特异性联.
- 观察到精细菌特异性CYP80基因扩张和组织特异性表达驱动BIA多样性.
结论:
- 整个基因组复制事件在早期分离的幼中在染色体进化中起着重要作用.
- CYP80基因的阴茎特异性基因扩张和表达模式是BIA多样性的关键驱动因素.
- 这项研究为理解Menispermaceae中BIA生物合成演变提供了一个模型.
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