萨尔加索姆种类 (Sargassum spp.) 是一种有毒的植物. 乙醇提取物引起斑马鱼 (Danio rerio) 胚胎的有毒反应和形
Carlos E González-Penagos1, Jesús Alejandro Zamora-Briseño2, Mónica Améndola-Pimenta1
1Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Mérida, Mérida, Yucatán, México.
Environmental toxicology and chemistry
|March 13, 2024
概括
加勒比海地区的藻过度生长造成了重大环境问题. 石灰提取物显著影响斑马鱼胚胎 (ZFEs),导致形态变化和改变基因表达,表明有毒性影响.
科学领域:
- 环境毒理学环境毒理学
- 海洋生物学 海洋生物学
- 生态毒理学 生态毒理学
背景情况:
- 增加了 Sargassum spp. 的数量. 加勒比海的海花对沿海生态系统构成环境威胁.
- 尤卡坦半岛受到萨尔加索姆洪水的特别影响,影响了生态动态.
研究的目的:
- 为了描述Sargassum spp.的毒理学影响. 斑马鱼胚胎 (ZFE) 上的乙醇提取物.
- 确定提取物中的关键化合物,并评估它们对ZFE发育和基因表达的影响.
主要方法:
- 在ZFEs使用Sargassum spp.进行了96小时的静态生物试验. 在不同度的乙醇提取物 (0.01400毫克/升).
- 气色谱质谱法 (GC-MS) 在提取物中确定了化合物.
- 用综合生物学反应 (IBRv2) 和总体形态评分 (GMS) 指数评估形态变化.
- 对ZFE暴露于200mg/L的提取物进行了比较性转录基因分析.
主要成果:
- 对ZFEs的中位数致命度 (LC50) 估计为251 mg/L.
- 观察到显著的形态异常,包括黄囊,脊椎病和色素损失.
- 转录组分析显示,暴露的ZFEs中66个上调调节和246个下调调节的基因.
- 上调的基因与外源生物反应和色素代谢有关,而下调的基因与神经系统和感官感知有关.
结论:
- 萨尔加索姆种类 (Sargassum spp.) 是一种有毒的植物. 提取物对斑马鱼胚胎具有显著的毒性.
- 观察到的形态和转录基因变化表明,体暴露对生命早期阶段产生了广泛的负面影响.
- 这项研究突出了藻花对海洋生物的潜在生态毒理风险.
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