纳米拓和口腔细菌在表面上的粘附:体外和体外研究
Humberto Osvaldo Schwartz-Filho1, Tauane Ramaldes Martins2, Paulo Roberto Sano2
1Universidade Federal do Paraná - UFPR, School, Department of Stomatology, Curitiba, PR, Brazil.
Brazilian oral research
|March 13, 2024
概括
在人体和实验室模型中,表面纳米拓没有显著影响初始细菌粘附. 这表明,从机械到纳米的表面质感差异不会改变植入物的早期细菌殖民.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 牙科植入物学 牙科植入物学
背景情况:
- 的表面地形对于牙植入成功至关重要.
- 了解细菌对植入物表面的粘附是预防围植入炎的关键.
- 纳米拓学在最初的细菌殖民中的作用需要进一步的研究.
研究的目的:
- 为了评估表面纳米拓对初始细菌粘附的影响.
- 为了比较细菌粘附 in vivo (口腔环境) 和 in vitro (实验室环境).
- 为了评估加工,微型和纳米拓图的表面的影响.
主要方法:
- 使用了具有不同地形 (加工,微,纳米) 的盘.
- 在体内实验:18名受试者24小时戴着带有磁盘的支柱.
- 在体外研究:与Streptococcus oralis和Actinomyces naeslundii.i.的粘附测试.
- 评估包括微生物培养,SEM和qPCR用于细菌量化.
主要成果:
- 在体内没有发现有氧或无氧细菌总数的显著差异.
- 在附着细菌中,qPCR没有显示出差异,除了在纳米拓学上较低的Actinomyces naeslundii.
- SEM显示所有群体的细菌分布都相似.
- 在体外研究表明,S. oralis和A. naeslundii的细菌粘附性没有差异.
结论:
- 微和纳米拓图没有显著影响最初的细菌粘附.
- 表面地形差异似乎没有影响的早期生物膜形成.
- 进一步的研究可能会探索更长期的影响或不同的细菌物种.
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