冠状病毒劫持了STX18-ATG14轴调节的脂质,以逃避抗病毒效应
Zhen Yuan1, Binbin Ding2,3
1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Autophagy
|March 13, 2024
概括
ATG14蛋白启动自并调解脂,即脂质滴的降解. STX18负面调节了这个过程,影响了病毒复制和像RSAD2.2这样的抗病毒蛋白的降解.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- ATG14是PtdIns3K-C1复合体的关键组成部分,对于启动宏自至关重要.
- ATG14还在脂质中发挥作用,即选择性降解脂质滴.
- 在自和脂路径中,STX18作为负调节器起作用.
研究的目的:
- 阐明ATG14在脂质代谢和病毒复制中的新作用.
- 研究STX18对ATG14介导的脂质的调节机制.
- 了解冠状病毒如何利用STX18来操纵脂质为自己的利益.
主要方法:
- 研究了ATG14,ATG8s和STX18.8之间的蛋白质与蛋白质相互作用.
- 利用STX18的淘汰来观察对脂质和RSAD2降解的影响.
- 检查了STX18在冠状病毒M蛋白诱导的脂质的作用.
主要成果:
- ATG14针对脂质滴并与ATG8相互作用以调节脂质.
- 通过与ATG14结合,STX18破坏了ATG14-ATG8相互作用和PtdIns3K-C1的形成.
- STX18 Knockdown增强了ATG14介导的脂质,导致RSAD2的降解.
- 冠状病毒M蛋白劫持STX18以诱导脂质和降解RSAD2,促进病毒复制.
结论:
- ATG14作为自受体,在脂质代谢和病毒复制中具有新的功能.
- STX18是ATG14介导的脂质的关键负调节剂.
- 针对STX18-ATG14轴为抗病毒疗法提供了潜在的策略.
相关概念视频
Receptor-mediated Endocytosis
6.1K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.1K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Immune Response Against Viral Pathogens
780
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
780
Mechanisms of Retrovirus-induced Cancers
5.1K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.1K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K


