一种松鼠SARS-CoV-2相关病毒分离物的特征,该分离物使用人类ACE2受体
Luo-Yuan Xia1,2, Xue-Feng Wang3, Xiao-Ming Cui2,4
1Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
Science China. Life sciences
|March 13, 2024
概括
一种新型的松鼠冠状病毒 (PCoV-GX/P2V) 可以感染人类细胞并引起小鼠的疾病. 目前的SARS-CoV-2免疫力可能无法防止这种病毒,强调需要进行监视.
科学领域:
- 病毒学 病毒学
- 动物传播疾病 动物传播疾病
- 比较病理学比较病理学
背景情况:
- 与SARS-CoV-2相关的冠状病毒经常在松鼠中发现,构成潜在的动物感染风险.
- 鱼是众所周知的各种冠状病毒的存储库,因此需要进一步研究它们的病原性潜力.
研究的目的:
- 为了调查与SARS-CoV-2相关的松鼠冠状病毒,PCoV-GX/P2V.的传染性和致病性.
- 评估PCoV-GX/P2V感染人类细胞并引起相关动物模型疾病的潜力.
主要方法:
- 使用人类细胞系 (肝瘤,结肠直肠腺癌,第一鼻上皮细胞) 的体外研究来评估病毒生长.
- 在体内实验涉及PCoV-GX/P2V的鼻腔注射到人类血管酶转化酶2 (hACE2) 转基因小鼠.
- 感染组织的组织病理学检查以表征疾病.
主要成果:
- PCoV-GX/P2V在人类细胞系中表现出感染性,包括表达HACE2的细胞系.
- 病毒在表达HACE2的细胞中有效复制,类似于SARS-CoV-2.
- 在 hACE2-转基因小鼠中,PCoV-GX/P2V引起了间歇性肺炎,炎症细胞透和鼻和肺中的气泡出血.
结论:
- PCoV-GX/P2V表现出显著的传染性和致病性,利用 hACE2受体进入.
- 这些发现表明,现有的对SARS-CoV-2的免疫力可能无法保护PCoV-GX/P2V.
- 密集监测对于监测和预防PCoV-GX/P2V对人类的潜在跨物种传播至关重要.
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