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Updated: Jun 18, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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在纹纳米颗粒上对膀癌微RNA进行无放大分析,其中含有DNA功能化量子点

Pei Wang1, Xiaowei Wei1, Luming Shen2

  • 1Laboratory Medicine Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China.

Analytical chemistry
|March 13, 2024
PubMed
概括

这项研究引入了一种使用纹纳米颗粒的新方法,用于用于膀癌 (BC) 诊断的微RNA (miRNA) 的敏感和特定多重检测. 该技术在没有放大的情况下实现了女性口腔敏感性,显示出临床应用的前景.

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...