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父母的热环境控制布鲁克 (Salvelinus fontinalis) 的后代表型:来自转录基因研究的见解
Ghizlane Banousse1, Eric Normandeau2, Christina Semeniuk3
1Institut des sciences de la mer de Rimouski (ISMER), Université du Québec à Rimouski (UQAR), Rimouski, QC, Canada G5L 2Z9.
G3 (Bethesda, Md.)
|March 13, 2024
概括
父母暴露于温度显著影响跨世代大脑基因表达. 温暖的父母温度改变了后代的神经和免疫基因表达,突出显示了气候变化适应风险.
科学领域:
- 水生生态学 水生生态学
- 环境表观遗传学环境表观遗传学
- 气候变化生物学 气候变化生物学
背景情况:
- 布鲁克查尔 (Brook charr) 是一种非常容易受到温度上升的影响的冷水鱼类.
- 环境变化可以诱导遗传的表型变化,可能通过表观遗传机制.
- 了解跨代效应对于预测物种对气候变化的反应至关重要.
研究的目的:
- 为了研究父母的热量制度和后代养殖温度如何影响布鲁克查尔的脑转录组.
- 评估基因表达的代际可塑性 (父母效应) 和代内可塑性 (后代环境).
主要方法:
- 布鲁克查尔的父母在生殖腺成熟期间暴露于寒冷或温暖的温度.
- 后代在5°C或8°C的温度下养.
- 使用Illumina NovaSeq6000在黄囊再吸收阶段对的脑转录组进行了测序.
主要成果:
- 在不同的后代养殖温度之间发现了很少的差异表达基因 (79).
- 在具有不同热史的父母的后代之间观察到大量的差异表达基因 (9,050).
- 与冷父母组相比,来自温暖父母组的弗莱显示了下调的神经和突触活动基因,但上调了DNA甲基化,新陈代谢,应激反应和免疫基因.
结论:
- 父母的热史对后代大脑基因表达有着深刻的影响,远远超过了直接养育温度的影响.
- 由DNA甲基化基因上调调节引起的表观遗传机制可能会调解这些跨代效应.
- 布鲁克查尔对气候变暖表现出复杂的分子反应,涉及神经功能变化和适应性生理调整,这对气候变化弹性有影响.
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