细胞增殖和Endoreduplication调节者的动态无处不在平衡决定了植物器官的大小
Ying Chen1,2, Mattias Vermeersch1,2, Jelle Van Leene1,2
1Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052 Ghent, Belgium.
Science advances
|March 13, 2024
概括
植物E3结合酶DA2的自基化调节了它的活性,而不是稳定性. 这一过程由UBP12/UBP13平衡,通过控制DA1酶活性来微调植物器官大小.
科学领域:
- 植物分子生物学 植物分子生物学
- 乌比奎丁-蛋白酶体系统
- 植物生长监管 植物生长监管
背景情况:
- 在植物发育过程中,ubiquitination至关重要.
- 在E3酶DA2激活酶DA1,限制细胞增殖.
- 调节DA2活动的机制以前是未知的.
研究的目的:
- 阐明调节E3酶DA2.2的分子机制.
- 为了研究DA2在植物生长中的自化作用.
- 了解DA2,DA1和脱化酶之间的相互作用.
主要方法:
- 调查DA2自化及其链接类型 (K48).
- 评估二氧化酶UBP12和UBP13对DA2的影响.
- 分析DA1蛋白酶活性对DA2介导的生长限制的影响.
- 利用合成的DA1-ubiquitin融合蛋白来研究生长调节.
主要成果:
- 通过K48链接,DA2经历了高周转率和自动无处不在,由UBP12和UBP13抵消.
- DA2 自主无化调节其 E3 酶活性,而不是蛋白质稳定性.
- 损坏的DA1蛋白酶活性会抵消DA2的增长抑制作用.
- 具有构成活性的DA1-ubiquitin融合严重限制了植物生长,并促进了Endoreduplication.
结论:
- DA2自主ubiquitination具有非蛋白质分解的功能,调节E3结合酶活性.
- DA2,UBP12和UBP13组成一个监管综合体,控制DA1的活动.
- 这种依赖于无处不在的机制精确地监测DA1活动,以微调植物器官大小.
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