由番茄CYP736A61催化的化合物的代谢
Thanh Dat Mai1, Hyun Min Kim1, Seo Young Park1
1School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
Enzyme and microbial technology
|March 13, 2024
概括
研究人员发现了一种新型番茄酶,Cytochrome P450 (CYP) 736A61,显示出独特的stilbene裂变活性. 这一发现促进了对植物代谢物生产和酶功能的理解.
科学领域:
- 植物生物化学 植物生物化学
- 酶学 是一种酶学.
- 代谢学 代谢学 代谢学
背景情况:
- 细胞染色体P450s (CYPs) 对于植物生长和应激反应至关重要,产生各种代谢物.
- 由于它们的结构相似,确定许多植物CYP的特定酶功能具有挑战性.
研究的目的:
- 为了分离和描述CYP736A61的酶功能,CYP736A61是番茄CYP736A亚家族的成员.
- 调查CYP736A61的基质特异性和反应机制.
主要方法:
- 在大肠杆菌中隔离和表达西红CYP736A61,使用与分子伴侣一起表达.
- 用各种基质进行重组酶和酶分析的净化,包括7-乙氧化,复星,聚达丁,皮诺斯蒂尔,,和佛洛.
主要成果:
- 纯化的CYP736A61呈现出对7-乙氧化的基化活性.
- 在resveratrol和polydatin上,CYP736A61的氧化活性较低.
- 最重要的是,CYP736A61调解了复星,聚达丁,皮诺斯蒂尔和斯特洛斯蒂尔的分裂.
- 该酶还将洛转化为纳灵宁,这表明了新型的stilbene裂变活性.
结论:
- CYP736A61是一种新型酶,具有显著的stilbene裂变活性.
- 这一发现扩大了植物细胞染色体P450酶的已知功能.
- 这项研究提供了关于植物中涉及stilbenes的代谢途径的见解.
相关概念视频
01:19Phase I Reactions: Oxidation of Aliphatic and Aromatic Carbon-Containing Systems
196
Phase I biotransformation reactions are integral to drug metabolism, predominantly involving oxidative, reductive, and hydrolytic transformations. Chief among these are oxidative reactions, which enhance the hydrophilicity of xenobiotics and introduce polar functional groups to facilitate their elimination from the body.
Oxidation reactions are fundamental in aromatic carbon-containing systems. An example is the hydroxylation of phenobarbital, a process that transforms it into...
Oxidation reactions are fundamental in aromatic carbon-containing systems. An example is the hydroxylation of phenobarbital, a process that transforms it into...
196
01:17Oxidation of Phenols to Quinones
3.0K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
3.0K
01:27Benzene to Phenol via Cumene: Hock Process
3.2K
The synthesis of phenol from benzene via cumene and cumene hydroperoxide is called the Hock process. First, a Friedel–Crafts alkylation reaction of benzene with propene gives cumene. Then cumene forms cumene hydroperoxide via a radical chain reaction. In the chain initiation step, the benzylic hydrogen is abstracted to give a benzylic radical. In the chain propagation step, the benzylic radical reacts with an oxygen diradical to form a cumene hydroperoxide radical. The cumene...
3.2K
01:14Drug Metabolism: Phase II Reactions
3.8K
Phase II reactions are essential for the detoxification and elimination of drugs from the body. These reactions involve the conjugation of parent drugs or their phase I metabolites with endogenous molecules, resulting in more hydrophilic drug conjugates. The primary conjugation reactions in this phase are sulfation and glucuronidation. Both sulfation and glucuronidation typically produce biologically inactive metabolites. However, in some cases involving prodrugs, active metabolites may be...
3.8K
01:17Drug Metabolism: Phase I Reactions
3.3K
A phase I reaction is a biochemical process that introduces a functionally reactive polar group to a substance. This transformation predominantly occurs in the liver, facilitated by the cytochrome P450 system of hemoproteins situated in the lipophilic endoplasmic reticulum of cells. The metabolite generated through this process can have varying polarities. If it is sufficiently polar, it can be easily excreted in the urine due to its water compatibility. However, if the metabolite is nonpolar,...
3.3K
01:19Phase I Oxidative Reactions: Overview
270
Phase I biotransformation, or functionalization, is a crucial chemical process that converts drugs and other xenobiotics into more water-soluble forms, facilitating expulsion from the body. It involves oxidative, reductive, and hydrolytic reactions that add or unveil polar functional groups on lipophilic substrates. Key players in phase I reactions are the mixed-function oxidases. Situated in liver cell microsomes, these enzymes predominantly carry out drug metabolism. They require molecular...
270