三醇杀菌剂对未暴露后代的基因表达和卵化合物的跨代影响:使用红腿 (Alectoris rufa) 的案例研究
Elena Fernández-Vizcaíno1, Rafael Mateo1, Isabel G Fernández de Mera1
1Instituto de Investigación en Recursos Cinegéticos (IREC) CSIC-UCLM-JCCM, Ronda de Toledo 12, 13005 Ciudad Real, Spain.
The Science of the total environment
|March 13, 2024
概括
被处理的种子中的triazole杀菌剂对鸟类构成风险,影响后代的繁殖. 这些内分泌干扰剂可以影响未暴露后代的基因表达和激素水平,突出显示长期的农药风险.
科学领域:
- 环境毒理学环境毒理学
- 鸟类生态学 鸟类生态学
- 内分泌学 在内分泌学.
背景情况:
- 三醇杀菌剂广泛用于谷物种子处理,通过摄入经过处理的种子,对食类鸟类 (如红腿 (Alectoris rufa)) 造成暴露风险.
- 三醇杀菌剂是已知的内分泌干扰剂,可以影响固醇合成和鸟类繁殖,具有跨代影响的潜力.
研究的目的:
- 调查三醇真菌杀菌剂暴露对红腿的生殖参数和基因表达的跨代影响.
- 评估三醇杀菌剂在多代暴露鸟类中的内分泌干扰作用的持续性.
主要方法:
- 成年红腿 (F0) 接触了用四种不同的三醇真菌杀菌剂配方处理的种子.
- 在蛋中分析了类固醇激素,胆固醇,维生素和胡卜素 (F1),在子中检查了与固醇生物合成相关的基因表达 (F1和F2).
主要成果:
- 在所有治疗组和两代 (F1和F2) 中都观察到涉及固醇生物合成的基因表达的变化.
- 具体的改变包括增加的雌激素水平 (flutriafol),降低的托科菲罗尔水平 (tebuconazole/prothioconazole混合物) 和增加的视网醇水平 (prothioconazole) 在F1代卵.
- 有证据表明,三醇杀菌剂的作用可以延伸到直接暴露之外,影响未暴露的后代.
结论:
- 暴露于三醇真菌杀菌剂可能会导致对鸟类生殖和内分泌功能的持续性,跨代影响.
- 这些发现强调了在评估农药对野生鸟类种群的影响时,需要考虑长期的慢性风险.
相关概念视频
02:27Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
00:49Complementation Tests
4.9K
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
4.9K