社会隔离导致肠道屏障障碍,并导致小鼠肠道微生物群的失衡
Yue Wang1, Hidayat Ullah1, Ting Deng1
1Department of Biotechnology, Dalian Medical University, Dalian, China.
Neuroscience letters
|March 13, 2024
概括
在小鼠中,社会隔离增加了焦虑,并损害了肠道屏障,改变了肠道微生物群的组成. 这些变化突出显示了肠-大脑轴.
科学领域:
- 神经科学是一个神经科学.
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 社会隔离是影响身体和精神健康的重要压力因素.
- 肠-大脑轴,涉及中枢神经系统和肠道微生物群之间的通信,是研究的一个关键领域.
- 肠道微生物可能会影响大脑功能和行为.
研究的目的:
- 研究社会隔离对肠道屏障和肠道微生物群的影响.
- 评估与小鼠社会隔离相关的行为变化.
主要方法:
- 40只雄性BALB/c小鼠被单独或分组安置了8周和15周.
- 测量了类似焦虑的行为.
- 评估了肠道屏障完整性 (紧密结合蛋白,粘蛋白表达).
- 在结肠组织中测量了炎症性细胞因子水平 (IL-6,IL-10,TNF-α).
- 使用Illumina MiSequencing分析了肠道微生物组成.
主要成果:
- 社会隔离的小鼠表现出类似焦虑的行为增加,随着时间的推移恶化.
- 在隔离8周和15周后,肠道机械和化学屏障的完整性下降.
- 在隔离15周后,炎症性细胞因子水平在结肠中增加.
- 肠道微生物群的组成发生了显著的变化,有益细菌水平降低,如Firmicutes, Bacteroides和Lactobacillus.
结论:
- 社会隔离会对肠道屏障功能产生负面影响,并促进炎症.
- 肠道微生物群组成的变化与社会隔离和类似焦虑的行为有关.
- 这些发现强调了肠-大脑轴在调解社会隔离的影响中的作用.
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