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一种模式识别受体 - - 介素-1受体 - - 参与Macrobrachium nipponense卵巢中的生殖免疫

Haifu Wan¹, Lei Yu², Xiaodong Cui²

¹College of Life Sciences, Hebei University, Baoding, China; Institute of Life Science and Green Development, Hebei University, Baoding, China; Hebei Innovation Center for Bioengineering and Biotechnology, Hebei University, Baoding, China; Postdoctoral Research Station of Biology, Hebei University, Baoding City, Hebei Province, 071002, China.

Fish & shellfish immunology

|March 13, 2024

概括

这项研究在东方河,Macrobrachium nipponense.中发现了一种新的互白素-1受体 (MnIL-1R). MnIL-1R识别了与病原体相关的分子模式,并激活了卵巢中的Toll信号通路.

科学领域:

免疫学免疫学免疫学
无脊椎动物动物学
分子生物学分子生物学

背景情况:

含有TIR域的受体对于天生的免疫是至关重要的.
介素-1受体 (IL-1R) 同类物存在于各种甲动物物种中.

研究的目的:

描述一个来自Macrobrachium nipponense卵巢的新型IL-1R (MnIL-1R).
研究MnIL-1R的保护和免疫功能.
阐明MnIL-1R在卵巢内的Toll信号通路中的作用.

主要方法:

打开阅读框架 (ORF) 分析和MnIL-1R.R的域识别.
定量实时PCR (qRT-PCR) 用于基因表达分析.
RNA干扰 (RNAi) 来评估MnIL-1R的功能.
结合测试以确定连接体的识别.

主要成果:

MnIL-1R具有1785bp的ORF编码594个氨基酸,具有保存的TIR和免疫球蛋白 (IG) 域.
在受到LPS,PGN和polyI:C的挑战时,MnIL-1R表达是广泛的,并在卵巢上升调节.
MnIL-1R的IG域与LPS和PGN结合,将其确定为模式识别受体.
在卵巢中,MnIL-1R沉默降低了关键的Toll信号通路基因 (Myd88,TRAF-6,Dorsal,Relish) 的调节.

结论:

MnIL-1R作为卵巢中的模式识别受体,通过其IG域识别LPS和PGN.
MnIL-1R激活了卵巢中的Toll信号通路,有助于Macrobrachium nipponense的天生的免疫力.

关键词:

马克罗布拉基乌尼波尼尼尼斯在MnIL-1RR中.卵巢卵巢卵巢模式识别受体是一种模式识别受体.

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