调节剂DegU可以显著影响性蛋白酶AprE在Bacillus licheniformis 2709中的生物合成
Cuixia Zhou1, Ying Kong2, Na Zhang2
1School of Biology and Brewing Engineering, Taishan University, Taian 271018, PR China; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science &Technology, Tianjin 300450, PR China.
International journal of biological macromolecules
|March 13, 2024
概括
调节器DegU对于Bacillus licheniformis中性蛋白酶AprE的产生至关重要. 化DegU直接与aprE调节区域结合,增强AprE生物合成和产量.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 来自Bacillus licheniformis的性蛋白酶AprE具有工业和医疗应用.
- AprE表达受到转录调节的严格控制.
- 了解AprE生物合成调节是改善菌株的关键.
研究的目的:
- 阐明性蛋白酶AprE生物合成在Bacillus licheniformis中的转录调节机制.
- 研究DegU/DegS双组件系统在AprE基因表达中的作用.
- 在aprE监管区域内确定DegU的DNA结合部位.
主要方法:
- 基因删除和 degU 和 degS 的补充.
- 电泳运动转移试验 (EMSA) 来评估蛋白质-DNA相互作用.
- 脱变体的等离子体介导过度表达.
- 假定DegU结合点的计算识别.
主要成果:
- 德格U和德格S的删除突变显示了严重减少的aprE表达.
- 化DegU在体外直接结合到aprE促进器区域.
- 过度表达酸化的DegU变体 (degU32(Hy)) 增加了41.6%的AprE产量.
- 假设的DegU结合序列在aprE转录起点的上游被确定.
结论:
- DegU/DegS系统,特别是化DegU,对于Bacillus licheniformis中的aprE转录至关重要.
- 酸化DegU通过与特定的DNA序列结合,直接调节aprE的表达.
- 这项研究提供了对AprE监管的见解,促进了未来的高产量战略.
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